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基本描述
| 产品名称 | THP-1 Whole Cell Lysate |
|---|---|
| 别名 | THP-1全细胞裂解物 | THP-1 全细胞裂解物 |
| 规格或纯度 | Mycoplasma free |
| 产品介绍 |
THP-1 is a monocyte isolated from peripheral blood from an acute monocytic leukemia patient. This cell line can be used in immune system disorder research, immunology research, and toxicology research.
|
储存与运输
| 物理形态 | 冻干(Lyophilized) |
|---|---|
| 储存缓冲液 | Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na₃VO₄). |
| 防腐剂 | No |
| 复溶 | Recommended reconstitute at 1-2 mg/mL in ddH₂O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. |
| 储存温度 | -80℃储存 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 用干冰运输。冻干粉建议在 -80℃ 下保存。复溶后，建议等分，避免反复冻融，短期保存在 -20℃ 下，长期保存在 -80℃ 下。 |
| 分子类型 | 细胞或组织提取物 |
图片
THP-1 Whole Cell Lysate (cl185703) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/2000 dilution
Lane 1: U- 2 OS Whole Cell Lysate (cl185708)
Lane 2: THP-1 Whole Cell Lysate (cl185703)
Lane 3: MOLT-4 Whole Cell Lysate (cl155905)
Lane 4: Jurkat Whole Cell Lysate (cl155897)
Lane 5: HeLa Whole Cell Lysate (cl155894)
Lane 6: THP-1 Whole Cell Lysate (cl185703)
Lane 7: HUVEC Whole Cell Lysate (cl185700)
Lane 8: TF-1 Whole Cell Lysate (cl185701)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 41 kDa
Exposure time: 53.8 s
THP-1 Whole Cell Lysate (cl185703) - Western Blot
All lanes: Recombinant PCNA Antibody (Ab120681) at 1/2000 dilution
Lane 1: U- 2 OS Whole Cell Lysate (cl185708)
Lane 2: THP-1 Whole Cell Lysate (cl185703)
Lane 3: MOLT-4 Whole Cell Lysate (cl155905)
Lane 4: Jurkat Whole Cell Lysate (cl155897)
Lane 5: HeLa Whole Cell Lysate (cl155894)
Lane 6: THP-1 Whole Cell Lysate (cl185703)
Lane 7: HUVEC Whole Cell Lysate (cl185700)
Lane 8: TF-1 Whole Cell Lysate (cl185701)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 31 kDa
Exposure time: 20.0 s
THP-1 Whole Cell Lysate (cl185703) - Western Blot
All lanes: Recombinant Hsp90 Antibody (Ab108929) at 1/2000 dilution
Lane 1: U- 2 OS Whole Cell Lysate (cl185708)
Lane 2: THP-1 Whole Cell Lysate (cl185703)
Lane 3: MOLT-4 Whole Cell Lysate (cl155905)
Lane 4: Jurkat Whole Cell Lysate (cl155897)
Lane 5: HeLa Whole Cell Lysate (cl155894)
Lane 6: THP-1 Whole Cell Lysate (cl185703)
Lane 7: HUVEC Whole Cell Lysate (cl185700)
Lane 8: TF-1 Whole Cell Lysate (cl185701)
Lysates at 20 µg per lane
SSecondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDa
Exposure time: 53.5 s
质检证书（CoA,COO,BSE/TSE 和分析图谱)
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