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基本描述
| 规格或纯度 | 适用于北方和南方的吸墨纸,粉末混合 |
|---|---|
| 英文名称 | Casein Blocking Buffer |
| 储存温度 | 室温 |
| 运输条件 | 常规运输 |
| 产品介绍 |
酪蛋白是一种牛奶来源的物质，通常用于 Western、Southern 和 Northern 印迹实验中的封闭缓冲液。封闭剂用于减少检测试剂与膜的非特异性结合。 应用 适合用作 Western、Southern 和 Northern 印迹膜的封闭缓冲液。然后，封闭膜可用于化学发光，比色或 DIG 检测分析。 适用性 原理 制备说明 将瓶中的全部内容物溶解在 800 mL 分子生物学级水中。搅拌直至充分混合，然后加水至终体积 1L。在 2-8°C 下最多保存一周。 外形 Casein is a milk-derived substance routinely used in blocking buffers for Western, Southern and Northern Blot procedures. Blocking agents are used to reduce non-specific binding of detection reagents to the membranes.Suitable as a blocking Buffer for Western, Southern and Northern Blot membranes. Blocked membranes can then be used in either chemiluminescent, colorimetric, or DIG detection assays. |
化学和物理性质
| 溶解性 | H2O：可溶，微黄色（在 5-10 分钟内溶解。） |
|---|
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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引用文献
| 1. Seiichi Katayama,Nanami Nozu,Masako Yokoyama,Yasuo Hitsumoto. (2006-12-27) Detection of fibronectin-binding proteins in Clostridium perfringens.. Acta medica Okayama, 60 ((6)): (351-355). [PMID:17189979] |
| 2. Mark D Bass,Mark R Morgan,Kirsty A Roach,Jeffrey Settleman,Andrew B Goryachev,Martin J Humphries. (2008-06-11) p190RhoGAP is the convergence point of adhesion signals from alpha 5 beta 1 integrin and syndecan-4.. The Journal of cell biology, 181 ((6)): (1013-1026). [PMID:18541700] |
| 3. Claire M Mahoney,Mark R Morgan,Andrew Harrison,Martin J Humphries,Mark D Bass. (2009-01-17) Therapeutic ultrasound bypasses canonical syndecan-4 signaling to activate rac1.. The Journal of biological chemistry, 284 ((13)): (8898-8909). [PMID:19147498] |
| 4. Elizabeth R Sunderhaus,Alexander D Law,Doris Kretzschmar. (2019-11-30) Disease-Associated PNPLA6 Mutations Maintain Partial Functions When Analyzed in Drosophila.. Frontiers in neuroscience, 13 (1207-1207). [PMID:31780887] |
| 5. Marlène Cassar,Alexander D Law,Eileen S Chow,Jadwiga M Giebultowicz,Doris Kretzschmar. (2020-04-16) Disease-Associated Mutant Tau Prevents Circadian Changes in the Cytoskeleton of Central Pacemaker Neurons.. Frontiers in neuroscience, 14 (232-232). [PMID:32292325] |
| 6. Xiaoli Ma,Brent E Stead,Atoosa Rezvanpour,Megan J Davey. (2010-09-24) The effects of oligomerization on Saccharomyces cerevisiae Mcm4/6/7 function.. BMC biochemistry, 11 (37-37). [PMID:20860810] |
| 7. Asser Nyander Poulsen,Inger Jansen-Olesen,Jes Olesen,Dan Arne Klaerke. (2010-10-13) Neuronal fast activating and meningeal silent modulatory BK channel splice variants cloned from rat.. Pflugers Archiv : European journal of physiology, 461 ((1)): (65-75). [PMID:20938677] |
| 8. Tadatsura Koshika,Carol Phelps,Jason Fang,Seung Eun Lee,Minoru Fujita,David Ayares,David K C Cooper,Hidetaka Hara. (2011-11-03) Relative efficiency of porcine and human cytotoxic T-lymphocyte antigen 4 immunoglobulin in inhibiting human CD4+ T-cell responses co-stimulated by porcine and human B7 molecules.. Immunology, 134 ((4)): (386-397). [PMID:22043861] |
| 9. S Tamir Rashid,Jonathan D Humphries,Adam Byron,Ameet Dhar,Janet A Askari,Julian N Selley,David Knight,Robert D Goldin,Mark Thursz,Martin J Humphries. (2012-06-15) Proteomic analysis of extracellular matrix from the hepatic stellate cell line LX-2 identifies CYR61 and Wnt-5a as novel constituents of fibrotic liver.. Journal of proteome research, 11 ((8)): (4052-4064). [PMID:22694338] |