碳酸酐酶

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COA

级别和纯度:

天然

EnzymoPure™

≥3,000 units/mg dry weight; from bovine erythrocytes

Accession #: Q1LZA1,P00921
生物活性: ≥3,000 units/mg dry weight

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货号 (SKU)	包装规格	是否现货
C128742-10mg	10mg	现货
C128742-50mg	50mg	现货
C128742-250mg	250mg	现货

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点击查看蛋白信息: CA5A 点击查看蛋白信息: CA5B 点击查看蛋白信息: CA6 点击查看蛋白信息: CA13 点击查看蛋白信息: CA1 点击查看蛋白信息: CA3 点击查看蛋白信息: CA2 点击查看蛋白信息: CA4 点击查看蛋白信息: CA12 点击查看蛋白信息: CA14 点击查看蛋白信息: CA9 点击查看蛋白信息: CA7

Accession#: P00921 (1) Accession#: Q1LZA1 (1) CA (1) CA1 (3) CA2 (3) Carbonic Anhydrase (40) Gene ID: 280740 (1) Gene ID: 510801 (1) Glucose Metabolism (3713) Metabolic Enzyme/Protease (4889) 酶 (62)

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基本描述

产品名称	碳酸酐酶
英文别名	Carbonate Dehydratase \| Carbonate Hydrolyase
规格或纯度	EnzymoPure™, 天然, ≥3,000 units/mg dry weight; from bovine erythrocytes
生化机理	碳酸酐酶是一种锌金属酶，分子量约为 30,000 Da。该酶催化二氧化碳水合为碳酸。它参与重要的生理和病理过程，如 pH 值和二氧化碳的平衡、碳酸氢盐和二氧化碳的运输、生物合成反应、骨吸收、钙化和肿瘤生成。因此，这种酶是抑制剂的一个重要靶点，临床应用于各种病症，如青光眼、癫痫和帕金森病。
产品介绍	基因名： CA1; CA2 基因 ID：510801; 280740 Carbonic anhydrase (CA) catalyzes the reversible hydration and dehydration reactions of CO2/H2CO3. CAs are widespread in nature, being found in animals, plants, and certain bacteria. Sixteen isozymes have been identified and characterized in mammals. Since erythrocyte CA is relatively easy to obtain for experimental purposes, it is the most widely studied. Specificity Blood CO2 transport and excretion is largely dependent on the rapid catalysis of the CO2 reactions within the erythrocyte by CA. Bovine CA reversibly hydrates alkyl pyruvates and it exhibits hydratase activity toward a wide variety of substrates. Composition Sixteen CA isozymes have been described so far in mammals. Erythrocyte CAs, CA-I and CA-II, are most well known. CA-I, CA-II, CA-III, CA-VII, and CA-XIII are cytosolic. CA-IV, CA-IX, CA-XII, CA-XIV, and CA-XV are membrane bound. CA-VI is secreted in saliva. CA-VA and CA-VB are mitochondrial. There are also three acatalytic forms referred to as CA-related proteins (CARPs): CARP-VIII, CARP-X, and CARP-XI. The zinc metal is always bound to histidines 93, 95, and 118 (mature chain numbering). A hydrogen bonded network, linked to the zinc-bonded water molecule and these histidines either directly or indirectly, includes 28-Ser, 91-Glu, 105-Glu, 106-His, 116-His, 193-Tyr, 198-Thr, 208-Trp, and 223-Asn. These residues have been found to be highly conserved. Bovine and human CA I and II contain a unique C-terminal knot structure, which has been shown to be important in enzymatic and mechanical properties. Molecular Characteristics Each isozyme from erythrocytes (CA-I and CA-II) is composed of a single chain peptide of 259 or 260 amino acid residues. The low activity form (CA-I) contains 260 residues, while the high activity form (CA-II) contains 259 residues. Erythrocyte high and low activity forms within a given species usually show greater than 50% sequence identity; for example, equine CA-I and CA-II forms only show 55% identity. In contrast, the same forms from different species show much greater homology; human CA-II and bovine CA-II show 77% sequence homology. The isozymes are encoded by separate genes but given the great deal of homology, especially in the active center, they appear to have a common evolutionary history. Ubiquitin, a 76 residue protein with some enzymatic properties of CAs, has a distinct sequence homology to CA. Applications CO2 determination in blood Elimination of CO2 in reagents for acidity testing Carboxy group transfers Reduction reactions Carbonic Anhydrase Assay Method The electrometric method of Wilbur and Anderson (1948) in which the time required (in seconds) for a saturated CO2 solution to lower the pH of 0.012 M Tris⋅HCl buffer from 8.3 to 6.3 at 0°C is determined. The time without enzyme is recorded at T0; with enzyme, T. Reagents 0.02 M Tris⋅HCl buffer, pH 8.0. Store in an ice bath at 0-4°C before and during use. Carbon dioxide saturated water. Bubble CO2 gas through 200 ml ice cold water for 30 minutes prior to assay. During saturation process, store water at 0-4°C in an ice bath. Enzyme Dissolve lyophilized powder at a concentration of 0.1 mg/ml in ice cold water. Store in ice bath prior to use. IMMEDIATELY prior to use dilute suspensions or lyophilized materials to a concentration of approximately 0.01 mg/ml in ice cold water. Procedure Blank Determination: Add 6.0 ml of chilled 0.02 M Tris⋅HCl buffer, pH 8.0 to a 15-20 ml beaker. Maintain temperature at 0-4°C and record pH. Withdraw in a 5 ml syringe, 4 ml of chilled CO2 saturated water and add to Tris buffer. Immediately start a stop watch and record the time required for the pH to drop from 8.3 to 6.3. Record this time as T0. Enzyme Determination: Add 6.0 ml of chilled 0.02 M Tris⋅HCl buffer, pH 8.0 to a 20 ml beaker. Maintain temperature at 0-4°C and record pH. Add 0.1 ml of freshly diluted enzyme. Quickly add 4 ml of CO2 saturated water and record the time required for the pH to drop from 8.3 to 6.3. Record this time as T. Calculation
生物活性	≥3,000 units/mg dry weight
种属	牛(Bovine)
Accession #	Q1LZA1,P00921
来源	天然

储存与运输

浓度	≥3,000 units/mg dry weight; from bovine erythrocytes
储存温度	2-8°C储存
运输条件	冰袋运输
CAS编号和信息	9001-03-0
酶学委员会编号	4.2.1.1
单位定义	One Unit is determined by the electrometric method of Wilbur and Anderson (J. Biol. Chem., 176, 147 (1948)), in which the time required (in seconds) for a saturated CO2 solution to lower the pH of 0.02M Tris-HCl buffer from 8.3 to 6.3, at 0-4°C is determi
分子类型	酶

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批号(Lot Number)	证书类型	货号
J2515302	分析证书	C128742
J2515340	分析证书	C128742
H2522772	分析证书	C128742
H2520660	分析证书	C128742
C2507322	分析证书	C128742
C2507323	分析证书	C128742
E2431092	分析证书	C128742
E2431104	分析证书	C128742
E2431091	分析证书	C128742
E2431090	分析证书	C128742
E2431093	分析证书	C128742
D2403171	分析证书	C128742
C2318945	分析证书	C128742
F2518195	分析证书	C128742
C2318947	分析证书	C128742
J2311119	分析证书	C128742
F2518172	分析证书	C128742
G2207196	分析证书	C128742
G2207197	分析证书	C128742
B2314433	分析证书	C128742

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引用文献

1. Meng-Meng Du, Yuan-Cheng Wang, Bao-Chang Sun, Yong Luo, Liang-Liang Zhang, Guang-Wen Chu, Hai-Kui Zou. (2025) Biocatalytic kinetics of the reaction between CO2 and tertiary amine using carbonic anhydrase. Chemical Engineering and Processing-Process Intensification, 211 (110218). [10.1016/j.cep.2025.110218]

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碳酸酐酶

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基本描述

储存与运输

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