基本描述

规格或纯度

BioReagent

稳定性与储存

Store at -20℃ long term (12 months).

英文名称

Biotin Assay Blocking Kit

储存温度

-20°C储存

运输条件

超低温冰袋运输

产品介绍

产品介绍：

1. 生物素检测封闭试剂盒(Biotin Assay Blocking Kit)，是一种主要用于SABC (Streptavidin-Biotin Complex)法或ABC (Avidin-Biotin Complex) 法等基于生物素和链霉亲和素 (Streptavidin) 或亲和素 (Avidin) 进行免疫组化(IHC)、免疫细胞化学(ICC)、免疫荧光(IF)、原位杂交(FISH)等检测过程中，对组织及细胞样品进行封闭以显著降低背景的试剂盒。

2. 本试剂盒含两种封闭液，Biotin Blocking Buffer (100X)中含有streptavidin等，可以充分结合并封闭内源性的生物素，并可以减少后续对于标记的streptavidin的非特异性结合；Streptavidin/Biotin-Binding Blocking Buffer (100X)中含有生物素，可以充分封闭内源性生物素结合蛋白以及前一步封闭时结合的streptavidin。通过两步法的封闭，可以达到显著降低背景的目的。

3. 本试剂盒适用于冰冻切片和石蜡切片的封闭，也适用于培养细胞的封闭。

产品特色：

1. 使用方便----可和常规的免疫染色封闭同时进行，显著节约封闭时间。

2. 效果稳定----有效减少非特异性染色，提高检测灵敏度。

产品组分及储存条件：

项目号	Component	200T	Storage
B1209118A	Biotin Blocking Buffer (100×)	200 μl	-20ºC
B1209118B	Streptavidin/Biotin-Binding Blocking Buffer (100×)	200 μl	-20ºC

操作步骤：

1．对于IHC、ICC、IF等免疫染色检测：

a. 封闭液的稀释：根据所需的用量，使用适当的常规封闭液稀释为1X的Biotin Blocking Buffer和Streptavidin/Biotin-Binding Blocking Buffer。

b. 在切片充分洗涤后，并且在一抗孵育之前，滴加适量Biotin Blocking Buffer，孵育10-30min。使用快速免疫染色封闭液进行稀释时，推荐孵育10min；使用普通封闭液进行稀释时，推荐孵育30min。

c. 洗涤液洗涤样品5min×3次。

d. 滴加适量Streptavidin/Biotin-Binding Blocking Buffer，孵育10-30min。使用快速免疫染色封闭液，推荐孵育10min；使用普通封闭液进行稀释时，推荐孵育30min。

e. 洗涤液洗涤样品5min×3次。

f. 按照常规步骤进行一抗孵育等后续操作。

2．对于原位杂交（FISH）检测：

a. 封闭液的稀释：根据所需的用量，使用适当的稀释液稀释为1X的Biotin Blocking Buffer和Streptavidin/Biotin-Binding Blocking Buffer。可用原位杂交的洗涤液进行稀释。

b. 在生物素标记探针杂交前，滴加适量Biotin Blocking Buffer，孵育15-30min。

c. 用洗涤液洗涤样品5min×3次。

d. 滴加适量Streptavidin/Biotin-Binding Blocking Buffer，孵育15-30min。

e. 用洗涤液洗涤样品5min×3次。

f. 按照常规的原位杂交检测步骤进行生物素标记探针杂交等后续操作。

注意事项:

1、本试剂盒也可以在一抗孵育完毕并用洗涤液洗涤3-4次后进行上述封闭步骤，随后再进行二抗孵育等步骤。

2、本试剂盒能同时进行生物素相关的封闭和常规封闭，无需再进行常规的免疫染色封闭操作步骤。

3、为了您的安全和健康，请穿实验服并戴一次性手套操作。

Product Introduction:
1. The Biotin Assay Blocking Kit is a kit primarily designed for blocking tissue and cell samples to significantly reduce background during detection processes such as immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), and fluorescence in situ hybridization (FISH). These detection methods are based on biotin and streptavidin or avidin, including the SABC (Streptavidin-Biotin Complex) method and ABC (Avidin-Biotin Complex) method.
2. This kit contains two types of blocking buffers: Biotin Blocking Buffer (100X): Contains streptavidin and other components, which can fully bind to and block endogenous biotin, and reduce non-specific binding to labeled streptavidin in subsequent steps. Streptavidin/Biotin-Binding Blocking Buffer (100X): Contains biotin, which can fully block endogenous biotin-binding proteins and the streptavidin bound during the previous blocking step. Through this two-step blocking process, the goal of significantly reducing background can be achieved.
3. This kit is suitable for blocking frozen sections and paraffin sections, and also applicable for blocking cultured cells.

Product Features:
1. Easy to Use – Can be performed simultaneously with the blocking step of conventional immunostaining, significantly saving blocking time.
2. Stable Performance – Effectively reduces non-specific staining and improves detection sensitivity.

Product Components and Storage Conditions:

Product number	Component	200T	Storage
B1209118A	Biotin Blocking Buffer (100×)	200 μl	-20ºC
B1209118B	Streptavidin/Biotin-Binding Blocking Buffer (100×)	200 μl	-20ºC

Operating Steps:
1. For immunostaining assays such as IHC, ICC, and IF:
a. Dilution of blocking buffers: Based on the required volume, dilute Biotin Blocking Buffer and Streptavidin/Biotin-Binding Blocking Buffer to 1X using an appropriate conventional blocking buffer.
b. After thorough washing of the sections and before primary antibody incubation, add an appropriate amount of Biotin Blocking Buffer and incubate for 10-30 minutes. Incubation for 10 minutes is recommended when diluted with a rapid immunostaining blocking buffer; incubation for 30 minutes is recommended when diluted with a regular blocking buffer.
c. Wash the samples with washing buffer 3 times, 5 minutes each.
d. Add an appropriate amount of Streptavidin/Biotin-Binding Blocking Buffer and incubate for 10-30 minutes. Incubation for 10 minutes is recommended when using a rapid immunostaining blocking buffer; incubation for 30 minutes is recommended when using a regular blocking buffer.
e. Wash the samples with washing buffer 3 times, 5 minutes each.
f. Proceed with subsequent steps such as primary antibody incubation according to conventional procedures.

2. For in situ hybridization (FISH) detection:
a. Dilution of blocking buffers: Based on the required volume, dilute Biotin Blocking Buffer and Streptavidin/Biotin-Binding Blocking Buffer to 1X using an appropriate diluent. The washing buffer for in situ hybridization can be used for dilution.
b. Before hybridization with biotin-labeled probes, add an appropriate amount of Biotin Blocking Buffer and incubate for 15-30 minutes.
c. Wash the samples with washing buffer 3 times, 5 minutes each.
d. Add an appropriate amount of Streptavidin/Biotin-Binding Blocking Buffer and incubate for 15-30 minutes.
e. Wash the samples with washing buffer 3 times, 5 minutes each.
f. Proceed with subsequent steps such as hybridization with biotin-labeled probes according to conventional in situ hybridization detection procedures.

Precautions:
1. This kit can also perform the above-mentioned blocking steps after the primary antibody incubation is completed and the sample is washed 3-4 times with washing buffer, followed by subsequent steps such as secondary antibody incubation.
2. This kit can simultaneously perform biotin-related blocking and conventional blocking, eliminating the need for additional conventional immunostaining blocking steps.
3. For your safety and health, please wear a lab coat and disposable gloves during operation.

名称和识别符

分子类型	试剂盒

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到1个结果

批号(Lot Number)	证书类型	货号
ZJ25F1028436	分析证书	B1209118

技术文档和文章

b1209118_生物素检测封闭试剂盒产品说明书.pdf

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

=

浓度

x

体积

x

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

x

体积 1 (V1)

=

浓度 2 (C2)

x

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

=

质量（小瓶）

÷

所需的复溶浓度

生物素检测封闭试剂盒

库存信息