Mouse Anti-Human IgG (AF594)

COA

级别和纯度:

已验证

ExactAb™

0.5mg/mL

应用:

Flow Cytometry
IF/ICC

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human)
亚型: Mouse IgG1
克隆号: 1H8
偶联: AF594

有货

库存信息

关闭

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货
Ab226740-10μg	10μg	现货
Ab226740-100μg	100μg	现货
Ab226740-500μg	500μg	现货

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AF594 (59) Mouse Anti-Human IgG (11)

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基本信息

产品名称	Mouse Anti-Human IgG (AF594)
别名	小鼠抗人IgG抗体 \| 小鼠抗人IgG \| 鼠抗人IgG抗体 \| 鼠抗人IgG
英文别名	Mouse Anti-Human IgG Antibody \| Mouse Anti Human IgG Antibody \| Anti-Human IgG Antibody \| Anti Human IgG Antibody \| Human IgG Antibody \| FLJ39988 antibodyFLJ40036 antibody \| FLJ40253 antibody \| FLJ40587 antibody \| FLJ40789 antibody \| FLJ40834 antibody \| G
规格或纯度	ExactAb™, 已验证, 0.5mg/mL
宿主种属	小鼠(Mouse)
特异性	Human IgG
种属反应性	人(Human)
交叉反应	No cross reactivity with mouse IgG, rabbit IgG and goat IgG.
免疫原	Human IgG
偶联	AF594
Excitation(nm)	Yellow-Green Laser(594nm)
Emission(nm)	618nm
Ex/Em(nm)	Ex:590nm;Em:618nm

产品属性

克隆类型	单克隆抗体
Format	Whole IgG
轻链亚型	kappa
纯化方法	Octylic acid-ammonium sulfate precipitation
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	0.5mg/mL
储存温度	2-8°C储存,避光,禁止冷冻
运输条件	冰袋运输
稳定性与储存	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
分子类型	抗体

图片

IF/ICC: Mouse Anti-Human IgG (AF594) (Ab226740)

Mouse Anti-Human IgG (AF594) (Ab226740) - IF/ICC
IF analysis of EpCAM (red) in HT-29 cells. The cells were fixed and permeabilized with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Citatuzumab (anti-EpCAM) (Ab209902) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Mouse Anti-Human IgG (AF594) (Ab226740) at a dilution of 1/500 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Mouse Anti-Human IgG (AF594) (Ab226740)

Mouse Anti-Human IgG (AF594) (Ab226740) - Flow Cytometry
Flow Cytometry analysis of HT-29 cells labelling EpCAM (red) with Citatuzumab (anti-EpCAM) (Ab209902). Mouse Anti-Human IgG (AF594) (Ab226740) at a dilution of 1/500 was used as the secondary antibody. Blue - Isotype control, Human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称	稀释比例
IF/ICC	1:500
Flow Cytometry	1/50 - 1/1000

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批号(Lot Number)	证书类型	货号
ZJ25F1028317	分析证书	Ab226740
ZJ25F1028316	分析证书	Ab226740
ZJ25F1028315	分析证书	Ab226740

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