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His tag (C-terminal) Mouse mAb (Biotin)

His tag (C-terminal) Mouse mAb (Biotin)

COA

级别和纯度:

已验证

ExactAb™

0.5 mg/mL

应用:

IF/ICC
WB

宿主种属: 小鼠(Mouse)
种属反应性: 不依赖种属(Species independent)
亚型: Mouse IgG1
克隆号: IJ08/4E6
偶联: Biotin

有货

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货号 (SKU)	包装规格	是否现货
Ab222055-10μl	10μl	现货
Ab222055-50μl	50μL	现货
Ab222055-100μl	100μL	现货
Ab222055-1ml	1ml	期货

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His tag (8)

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基本信息

产品名称	His tag (C-terminal) Mouse mAb (Biotin)
别名	聚组氨酸标签单克隆抗体 \| 聚组氨酸抗体 \| His tag标签抗体 \| His tag单克隆抗体 \| 6xHis抗体 \| His标签 \| His标签抗体 \| His标签小鼠单克隆抗体 \| His抗体
英文别名	6 His epitope tag antibody \| Hexa His tag antibody \| HHHHHH epitope tag antibody \| HHHHHH tag antibody \| His tag antibody \| Polyhistidine Tag antibody \| His tag Mouse Monoclonal Antibody \| His tag Mouse mAb \| Anti-His tag \| Monoclonal Anti- His tag antibo
规格或纯度	ExactAb™, 已验证, 0.5 mg/mL
生化机理	The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
宿主种属	小鼠(Mouse)
特异性	Recombinant proteins containing the 6X His tag® fused to carboxy terminus of fusion proteins.
种属反应性	不依赖种属(Species independent)
免疫原	A recombinant protein containing the sequence HHHHHH
阳性对照	WB: His-transfected HEK293 whole cell lysates; C-terminal His fusion proteins
偶联	Biotin

产品属性

克隆类型	单克隆抗体
Format	Whole IgG
轻链亚型	kappa
纯化方法	Protein A purified
储存缓冲液	Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	0.5 mg/mL
储存温度	避光,-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
分子类型	抗体

图片

Western Blot: His tag (C-terminal) Mouse mAb (Biotin) (Ab222055)

His tag (C-terminal) Mouse mAb (Biotin) (Ab222055) - Western Blot
All lanes: His tag (C-terminal) Mouse mAb (Biotin) (Ab222055) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: SA-HRP (np156148) at 1/10000 dilution

Predicted band size: 113 kDa
Observed band size: 52, 113 kDa
Exposure time: 120.0 s

应用

应用名称	稀释比例
WB	1/1000 - 1/10000
IF/ICC	1/100-1/1000

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0724169	分析证书	Ab222055
ZJ25F0724168	分析证书	Ab222055
ZJ25F0724167	分析证书	Ab222055

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