Goat Anti-Human IgG (LumiDye™ Blue 575)

COA

级别和纯度:

已验证

ExactAb™

1.0 mg/mL

应用:

Flow Cytometry
IF/ICC

宿主种属: 山羊(Goat)
种属反应性: 人(Human)
亚型: Goat IgG
偶联: LumiDye™ Blue 575

有货

库存信息

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库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
Ab220543-10μg	10μg	现货
Ab220543-100μg	100μg	现货
Ab220543-1mg	1mg	现货

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Goat Anti-Human IgG (27) LumiDye™ Blue 575 (3)

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基本信息

产品名称	Goat Anti-Human IgG (LumiDye™ Blue 575)
别名	羊抗人IgG抗体 \| 羊抗人IgG \| 山羊抗人IgG \| 山羊抗人IgG抗体
英文别名	Goat Anti-Human IgG H&L Antibody \| Goat Anti Human IgG H&L Antibody \| Anti-Human IgG H&L Antibody \| Anti Human IgG H&L Antibody \| Human IgG H&L Antibody \| Goat Anti-Human IgG Antibody \| Goat Anti Human IgG Antibody \| Anti-Human IgG Antibody \| Anti Human I
规格或纯度	ExactAb™, 已验证, 1.0 mg/mL
宿主种属	山羊(Goat)
种属反应性	人(Human)
偶联	LumiDye™ Blue 575
Excitation(nm)	Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)	575nm
Ex/Em(nm)	Ex:565nm;Em:575nm

产品属性

克隆类型	多克隆抗体
Format	Whole IgG
纯化方法	antigen affinity chromatography
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	1.0 mg/mL
储存温度	2-8°C储存,避光,禁止冷冻
运输条件	冰袋运输
稳定性与储存	The product should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
分子类型	抗体

图片

IF/ICC : Goat Anti-Human IgG (LumiDye™ Blue 575) (Ab220543)

Goat Anti-Human IgG (LumiDye™ Blue 575) (Ab220543) -IF/ICC
IF analysis of EpCAM (red) in HT-29 cells. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Citatuzumab (anti-EpCAM) (Ab209902) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Human IgG (LumiDye™ Blue 575) (Ab220543) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Goat Anti-Human IgG (LumiDye™ Blue 575) (Ab220543)

Goat Anti-Human IgG (LumiDye™ Blue 575) (Ab220543) - Flow Cytometry
Flow Cytometry analysis of HT-29 cells labelling EpCAM (red) with Citatuzumab (anti-EpCAM) (Ab209902). Goat Anti-Human IgG (LumiDye™ Blue 575) (Ab220543) at a dilution of 1/900 was used as the secondary antibody. Blue - Isotype control, Human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称	稀释比例
IF/ICC	1/100-1/1000
Flow Cytometry	1/100-1/1000

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批号(Lot Number)	证书类型	货号
ZJ25F0724009	分析证书	Ab220543
ZJ25F0724010	分析证书	Ab220543
ZJ25F0724008	分析证书	Ab220543

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