基本描述

别名	漂片抗原修复液
英文别名	Antigen Retrieval Solution (10X)
规格或纯度	BioReagent, 无菌过滤, 适用于免疫组织化学, 10X
稳定性与储存	Store at 2-8℃ for 12 months. Store at -20℃ for 24 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
英文名称	Antigen Retrieval Solution for Floating Sections (10×)
储存温度	2-8°C储存
运输条件	冰袋运输
产品介绍	漂片抗原修复液(10×)含有柠檬酸钠等抗原修复试剂等，pH值约为8.5，可有效降低漂片破碎的风险并去除醛类固定试剂导致的蛋白之间的交联，充分暴露石蜡切片等样品中的抗原表位，从而大大改善免疫染色效果。其适用于漂片的抗原修复，也可以用于石蜡切片、冰冻切片等样品的抗原修复。操作流程： 1、试剂准备使用前必须用蒸馏水或超纯水将漂片抗原修复液(10×)稀释10倍，配制成抗原修复液(1×)。 2、抗原修复 2.1石蜡切片： 2.1.1脱蜡：切片在二甲苯中脱蜡2×5min。无水乙醇2×5min。90%乙醇2×5min，70％乙醇5min。蒸馏水2×5min。 2.1.2抗原修复：将切片浸泡在抗原修复液(1×)中，80°C加热约30min(最佳的加热时间需根据不同的样品和目的蛋白自行摸索)。注：抗原修复液(1×)使用前需预热到80°C。可以使用水浴锅加热。随后大约在20~30min冷却至室温。用免疫染色洗涤液洗涤1~2次，每次3~5min。即可进行封闭等后续的免疫染色步骤。 2.2冰冻切片：用免疫染色洗涤液洗涤切片5min。抗原修复的过程如石蜡切片的抗原修复过程。 2.3对于其它样品的抗原修复，可以参考石蜡切片或冰冻切片的步骤进行。温馨提示：为了您的自身安全，使用试剂前，请做好防护，如穿实验服，带手套等。 Antigen Retrieval Solution for Floating Sections (10×) contains sodium citrate and other antigen repair reagents, pH value is about 8.5, can effectively reduce the risk of bleach breakage and remove the cross-linking between proteins caused by aldehyde fixing reagents, fully expose the antigen epitope in paraffin sections and other samples, thus greatly improving the immunostaining effect. It is suitable for the antigen repair of bleaching pieces, and can also be used for the antigen repair of paraffin sections, frozen sections and other samples. Operation process: 1. Before the reagent is ready to use, the antigen repair solution (10×) must be diluted 10 times with distilled water or ultra-pure water to prepare the antigen repair solution (1×). 2. Antigen Repair 2.1 Paraffin section: 2.1.1 Dewaxing: section dewaxing in xylene for 2×5min. Anhydrous ethanol 2×5min. 90% ethanol 2×5min, 70% ethanol 5min. Distilled water 2×5min. 2.1.2 Antigen repair: The section is immersed in antigen repair solution (1×) and heated at 80°C for about 30min(the optimal heating time should be determined according to different samples and target proteins). Note: Antigen repair solution (1×) should be preheated to 80°C before use. Can be heated in a water bath. Then cool to room temperature in about 20 to 30 minutes. Wash with immunostaining detergent 1~2 times, 3~5min each time. Subsequent immunostaining steps such as blocking can be performed. 2.2 Frozen section: The section was washed with immunostaining detergent for 5min. The process of antigen repair such as the process of antigen repair in paraffin sections. 2.3 For antigen repair of other samples, please refer to the steps of paraffin section or frozen section. Tips: For your own safety, please take good protection before using the reagent, such as wearing a lab coat, wearing gloves, etc.

产品属性

pH	8.5

名称和识别符

分子类型	生物试剂/缓冲液

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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批号(Lot Number)	证书类型	货号
ZJ25F0420219	分析证书	A752121

技术文档和文章

a752121_漂片抗原修复液10x 产品说明书.pdf

a752121_ antigen retrieval solution for floating sections product manual.pdf

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

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稀释计算器

计算配制储备溶液所需的稀释度。

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浓度 2 (C2)

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复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

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所需的复溶浓度

漂片抗原修复液（10X）

库存信息