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Aladdin® 594 NHS 酯(琥珀酰亚胺酯)

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库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
A729269-1mg
1mg 现货 Stock Image
A729269-5mg
5mg 现货 Stock Image
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荧光染料 (60)

基本描述

规格或纯度 Ex:590nm, Em:618nm, mixture of isomers
稳定性与储存 存放在冷冻室(-20 ℃)并避光。
英文名称 Aladdin® 594 NHS Ester (Succinimidyl Ester)
储存温度 避光,-20°C储存,干燥,避免反复冻融
运输条件 超低温冰袋运输
产品介绍


Aladdin® 594 is a bright red dye. Used for stable signal generation in imaging and flow cytometry, Aladdin® 594 dye is water soluble and pH-insensitive from pH 4 to pH 10. In addition to reactive dye formulations, we offer Aladdin® 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. 

The NHS ester (or succinimidyl ester) of Aladdin® 594 is the most popular tool for conjugating this dye to a protein or antibody. NHS esters can be used to label to the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting Aladdin® conjugate will exhibit brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores. 

Detailed information about this Aladdin® NHS ester:

Fluorophore label: Aladdin® 594 dye

Reactive group: NHS ester

Reactivity: Primary amines on proteins and ligands, amine-modified oligonucleotides

Ex/Em of the conjugate: 590/617 nm

Extinction coefficient: 92,000 cm-1M-1

Molecular weight: 819.8

The Aladdin® NHS ester is typically dissolved in high-quality anhydrous dimethylformamide (DMF) or dimethylsulfoxide (DMSO), and the reaction is carried out in 0.1–0.2 M sodium bicarbonate buffer, pH 8.3, at room temperature for 1 hour. Because the pKa of the terminal amine is lower than that of the lysine epsilon-amino group, you may achieve more selective labeling of the amine terminus using a buffer closer to neutral pH.

名称和识别符

分子量 819.85(free base)

化学和物理性质

溶解性 soluble in water, DMSO, DMF
敏感性 对光敏感

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批号(Lot Number) 证书类型 货号
B2526242 分析证书 A729269
B2526243 分析证书 A729269
B2526244 分析证书 A729269
B2526245 分析证书 A729269

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