Aladdin Fluor 790 NHS Ester

COA

级别和纯度:

Ex:784nm

Em:814nm

有货

库存信息

关闭

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货
A638848-100μg	100μg	期货
A638848-1mg	1mg	现货
A638848-5mg	5mg	现货

查看相关系列

荧光染料 (60) 蛋白标记试剂 (108)

Skip to the end of the images gallery

Skip to the beginning of the images gallery

基本描述

规格或纯度	Ex:784nm, Em:814nm
英文名称	Aladdin Fluor 790 NHS Ester
储存温度	避光,-20°C储存
运输条件	超低温冰袋运输
产品介绍	Product introduction： Alexa Fluor™ 790 is a bright and photostable near-IR dye that is spectrally similar to indocyanine green (ICG) and the IRDye™ 800 dye. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor™ 790 dye is water soluble and pH-insensitive from pH 4 to pH 10. Fluorescence of this long-wavelength Alexa Fluor™ dye is not visible to the human eye but is readily detected by most imaging systems. As the longest-wavelength Alexa Fluor™ dye, the emission is well separated from commonly used far-red fluorophores such as Alexa Fluor™ 647 dye or allophycocyanin (APC), facilitating multicolor analysis. This fluorophore is also expected to be useful for small animal in-vivo imaging (SAIVI) applications or for two-color western applications with the LI-COR™ Odyssey™ infrared imaging system. In addition to reactive dye formulations, we offer Alexa Fluor™ 790 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection.The NHS ester (or succinimidyl ester) of Alexa Fluor™ 790 is the most popular tool for conjugating this dye to a protein or antibody. NHS esters can be used to label to the primary amines (R-NH₂) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting Alexa Fluor™ conjugate will exhibit brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores. Detailed information about this Aladdin Fluor NHS ester: Fluorophore label: Alexa Fluor™ 790 dye Reactive group: NHS ester Reactivity: Primary amines on proteins and ligands, amine-modified oligonucleotides Ex/Em of the conjugate: 784/814 nm Extinction coefficient: 260,000 cm-1M-1 Spectrally similar dyes: indocyanine green (ICG) and the IRDye™ 800 dye Molecular weight: ∼1750 Typical Conjugation Reaction You can conjugate amine-reactive reagents with virtually any protein or peptide (the provided protocol is optimized for IgG antibodies). You can scale the reaction for any amount of protein, but the concentration of the protein should be at least 2 mg/mL for optimal results. We recommend trying three different degrees of labeling, using three different molar ratios of the reactive reagent to protein. The Alexa Fluor™ NHS ester is typically dissolved in high-quality anhydrous dimethylformamide (DMF) or dimethylsulfoxide (DMSO) , and the reaction is carried out in 0.1–0.2 M sodium bicarbonate buffer, pH 8.3, at room temperature for 1 hour. Because the pKa of the terminal amine is lower than that of the lysine epsilon-amino group, you may achieve more selective labeling of the amine terminus using a buffer closer to neutral pH. Conjugate Purification Labeled antibodies are typically separated from free Alexa Fluor™ dye using a gel filtration column, such as Sephadex™ G-25, BioGel™ P-30, or equivalent. For much larger or smaller proteins, select a gel filtration media with an appropriate molecular weight cut-off or purify by dialysis. We offer several purification kits optimized for different quantities of antibody conjugate: Antibody Conjugate Purification Kit for 0.5-1 mg () Antibody Conjugate Purification Kit for 20-50 µg () Antibody Conjugate Purification kit for 50-100 µg () Learn More About Protein and Antibody Labeling We offer a wide selection of Molecular Probes™ antibody and protein labeling kits to fit your starting material and your experimental setup. See ourAntibody Labeling kits or use ourKits for Labeling Proteins and Nucleic Acids—Section 1.2 in The Molecular Probes™ Handbook. We’ll Make a Custom Conjugate for You If you can’t find what you’re looking for in our online catalog, we’ll prepare a custom antibody or protein conjugate for you. Ourcustom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.

化学和物理性质

敏感性	易吸潮
Excitation(nm)	784
Emission(nm)	814

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到4个结果

批号(Lot Number)	证书类型	货号
E2529236	分析证书	A638848
E2529237	分析证书	A638848
E2529263	分析证书	A638848
E2529264	分析证书	A638848

引用文献

1. Yang J, Singh V, Cha B, Chen TE, Sarker R, Murtazina R, Jin S, Zachos NC, Patterson GH, Tse CM, Kovbasnjuk O, Li X, Donowitz M,. NHERF2 protein mobility rate is determined by a unique C-terminal domain that is also necessary for its regulation of NHE3 protein in OK cells.. J Biol Chem, [PMID:23612977]
2. Dempsey GT, Vaughan JC, Chen KH, Bates M, Zhuang X,. Evaluation of fluorophores for optimal performance in localization-based super-resolution imaging.. Nat Methods, [PMID:22056676]

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

Aladdin Fluor 790 NHS Ester

库存信息

库存信息

库存信息

基本描述

产品属性

化学和物理性质

质检证书（CoA,COO,BSE/TSE 和分析图谱)

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器