基本描述

规格或纯度

BioReagent

稳定性与储存

各组分在相应的储存条件下保质期1年。

英文名称

Protein Labeling Kit (AIE)

储存温度

2-8°C储存,避光,室温,-20°C储存

运输条件

超低温冰袋运输

产品介绍

蛋白标记试剂盒（聚集诱导发光）是基于聚集诱导发光（AIE）原理开发的一款用于标记一般蛋白的试剂盒。其标记原理是，试剂盒中的AIE染料携带活性基团，可以与蛋白表面的一级氨基（赖氨酸残基）发生反应形成稳定的酰胺键。AIE染料具有较高的反应活性，可对一般蛋白进行快速标记。

AIE染料的最佳激发波长：460 nm，发射波长范围：570‒700 nm，适用于配备了蓝光激发波长检测器的系列仪器。

产品组分表：

A1456399	Component	1套	Storage
A1456399A	AIE染料冻干粉	1管	-20℃. Store in the dark.
A1456399B	Buffer (1×)	30 mL	2-8℃.
A1456399C	0.1μm 针头过滤器	10个	RT.

标记效果图：

备注：

1、客户收到产品后请按产品标签储存温度保存。

2、客户需要自备二甲基亚砜（DMSO）试剂，一次性注射器耗材。

实验方法

1．蛋白样品缓冲溶剂的置换在开始标记蛋白之前，请确保蛋白样品缓冲液与试剂盒兼容。如果您的蛋白样品缓冲液种含有一级氨基（比如Tris和glycine）、咪唑、DTT、β-巯基乙醇中的一种或多种，请使用Buffer 1×缓冲溶液进行置换后再标记。

2．AIE染料储备液配制：向AIE染料冻干粉小管加入20μL体积DMSO混匀溶解，配制成浓度为10 mM的AIE染料储备液。

备注：1) DMSO溶解前AIE染料冻干粉先恢复至室温后离心10s。

2) 溶解后可按需求分装保存。

3. 蛋白标记方法（推荐）

1)用Buffer 1×将蛋白样品稀释为浓度约为150 μg/mL。

2)向上述蛋白样品溶液中加入10 mM的AIE染料储备液，使其在蛋白样品溶液中的终浓度为10μM（比如，蛋白样品溶液体积为1mL时，加入1 μL的10 mM的AIE染料储备液，可根据蛋白样品的实际情况调整用量），混匀。

3)室温避光孵育5~10 min。

4)标记结束后，用0.1 μm针头过滤器过滤标记溶液，以去除多余AIE染料。

注意事项

1．AIE染料对湿度敏感，请确保在-20°C条件下干燥避光储存。配制储备液前，请将染料恢复至室温，并离心10s。配制成储备液后，请分装保存并尽快使用。

2．试剂盒中所提供的缓冲液（pH = 8.5）是AIE染料工作的首选，您可以选择任意一种不含有一级氨基、咪唑、DTT或β-巯基乙醇的缓冲液进行替代，但可能会影响标记效率。

3．建议标记时蛋白的最低浓度不低于15 μg/mL。

4．因蛋白表面的氨基数量和反应活性不同，不同蛋白的标记效果可能存在差异。如果标记效果不佳，可适当提高染料标记浓度或延长反应时间。

5．为了您的安全和健康，请穿实验服并戴一次性手套操作。

6．本品仅适用于科研用途。

Protein Labeling Kit (Aggregation-induced emission, AIE) is developed based on the principle of aggregation-induced emission (AIE) and is used for labeling general proteins. Its labeling principle is that the AIE dye in the kit carries active groups, which can react with the primary amino groups (lysine residues) on the protein surface to form stable amide bonds. The AIE dye has high reactivity and can quickly label general proteins.
The optimal excitation wavelength of the AIE dye is 460 nm, and the emission wavelength range is 570–700 nm, which is suitable for a series of instruments equipped with a blue light excitation wavelength detector.

Marking renderings:

Notes:

After receiving the product, customers should store it according to the temperature indicated on the product label.
Customers need to prepare dimethyl sulfoxide (DMSO) reagents and disposable syringe consumables by themselves.

Experimental Methods

1.Replacement of protein sample buffer solvent: Before starting to label the protein, ensure that the protein sample buffer is compatible with the kit. If your protein sample buffer contains one or more of primary amino groups (such as Tris and glycine), imidazole, DTT, and β-mercaptoethanol, please replace it with Buffer 1× buffer solution before labeling.

2.Preparation of AIE dye stock solution: Add 20 μL of DMSO to the small tube containing AIE dye lyophilized powder, mix well to dissolve, and prepare a 10 mM AIE dye stock solution.
Notes: 1) Before dissolving with DMSO, let the AIE dye lyophilized powder return to room temperature and centrifuge for 10 seconds.

2) After dissolution, it can be aliquoted for storage as needed.

3.Protein labeling method (recommended)

1) Dilute the protein sample with Buffer 1× to a concentration of approximately 150 μg/mL.

2) Add 10 mM AIE dye stock solution to the above protein sample solution to make its final concentration in the protein sample solution 10 μM (for example, when the volume of the protein sample solution is 1 mL, add 1 μL of 10 mM AIE dye stock solution; the dosage can be adjusted according to the actual situation of the protein sample), and mix well.

3) Incubate at room temperature in the dark for 5–10 minutes.

4) After labeling, filter the labeled solution with a 0.1 μm needle filter to remove excess AIE dye.
Precautions

The AIE dye is sensitive to humidity, so ensure that it is stored at -20°C in a dry and dark place. Before preparing the stock solution, let the dye return to room temperature and centrifuge for 10 seconds. After preparing the stock solution, aliquot it for storage and use it as soon as possible.
The buffer solution (pH = 8.5) provided in the kit is the first choice for the AIE dye to work. You can choose any buffer solution that does not contain primary amino groups, imidazole, DTT, or β-mercaptoethanol as a substitute, but it may affect the labeling efficiency.
It is recommended that the minimum concentration of the protein during labeling is not less than 15 μg/mL.
Due to the differences in the number and reactivity of amino groups on the protein surface, the labeling effects of different proteins may vary. If the labeling effect is not good, the dye labeling concentration can be appropriately increased or the reaction time can be prolonged.
For your safety and health, please wear a lab coat and disposable gloves during operation.
This product is only for scientific research purposes.

化学和物理性质

敏感性	Hygroscopic

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到1个结果

批号(Lot Number)	证书类型	货号
ZJ25F1028734	分析证书	A1456399

技术文档和文章

a1456399_蛋白标记试剂盒聚集诱导发光产品说明书.pdf

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

=

浓度

x

体积

x

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

x

体积 1 (V1)

=

浓度 2 (C2)

x

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

=

质量（小瓶）

÷

所需的复溶浓度

蛋白标记试剂盒（聚集诱导发光）

库存信息