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Western Antibody Dilution Buffer

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Western Antibody Dilution Buffer

3 Citations

COA

Grade & Purity:

for molecular biology

Suitable for molecular biology

In stock

Item Number

W666033

Grouped product items
SKU	Size	Availability	Price	Qty
W666033-100ml	100ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$54.90
W666033-500ml	500ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$169.90

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Basic Description

Synonyms	Antibody Dilution Solution \| Antibody Dilution Buffer \| Universal antibody diluent \| Primary and Secondary antibody diluent \| Diluted solution of primary and secondary antibodies
Specifications & Purity	sterile, BioReagent, Suitable for molecular biology, for western blot, Suitable for Immunohistochemistry, Suitable for Immunofluorescence, ready-to-use
Stability And Storage	Store at 2-8℃ for 12 months; Store at -20℃ for 24 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads This product requires cold chain shipping. Ground and other economy services are not available.
Grade	BioReagent, for molecular biology, for western blot, ready-to-use, sterile, Suitable for molecular biology, 废弃for biotechnology
Product Description	Product Introduction: The Universal antibody diluent is optimized through repeated optimization of an appropriate amount of BSA, buffer, detergent, polymer, and non competitive protein to form a protective effect on the antibody protein and improve the specific binding of the antibody to the target molecule. At the same time, it is also beneficial to prevent non-specific binding between antibodies and membranes or non target proteins, and effectively improve the stable storage time of diluted primary antibodies. Precautions： 1. This product contains BSA. If bacterial growth occurs, please stop using it. 2. For your safety and health, please wear lab clothes and disposable gloves when operating. Instructions for Use： Dilute the corresponding primary and secondary antibodies with Universal antibody diluent according to the recommended conditions in the antibody manual. For example, dilute at a ratio of 1:1000 by adding 1 µL of antibody to 1000 µ L of Universal antibody dilution solution.

Names and Identifiers

PH	7.0～7.6

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Alternative Products

Documents & Articles

w666033_ universal antibody dilution buffer product manual.pdf

Western Blotting with Fluorescently Labeled Antibodies

Categories: Protocols

Tags:

Protein Electrophoresis & Western Blotting Biological buffers Immunoblotting Fluorescently Labeled Antibodies Membrane Transfer

Citations of This Product

How to Cite Aladdin Scientific Products?

1. Jin Cui, Gongzheng Wang, Li Xian Yip, Mengzhen Dong, Mengyao Mu, Liya Tian, Yuan Gao, Qing Fan, Qiang Zhu, Xinya Zhao, Xueli Xu, David Tai Leong, Xiao Sun. (2024) Enhanced Ferritin-Manganese Interaction by Nanoplatinum Growth Enabling Liver Fibrosis 3D Magnetic Resonance Visualization and Synergistic Therapy with Real-Time Monitoring. ADVANCED FUNCTIONAL MATERIALS, (2410748).
2. Yuan Gao, Mengyao Mu, Yiju Wei, Bowen Yan, Hui Liu, Kai Guo, Mengmeng Zhang, Xiaohui Dai, Xiao Sun, David Tai Leong. (2025) Novel ultrathin ferrous sulfide nanosheets: Towards replacing black phosphorus in anticancer nanotheranostics. Bioactive Materials, 43 (564).
3. Guiqi Ma, Xinyu Zhang, Kunlong Zhao, Shuxuan Zhang, Ke Ren, Mengyao Mu, Chenyu Wang, Ximing Wang, Hui Liu, Jian Dong, Xiao Sun. (2024) Polydopamine Nanostructure-Enhanced Water Interaction with pH-Responsive Manganese Sulfide Nanoclusters for Tumor Magnetic Resonance Contrast Enhancement and Synergistic Ferroptosis–Photothermal Therapy. ACS Nano, 18 (4): (3369-3381).

Solution Calculators

Molarity Calculator

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*When preparing stock solutions, always use the batch-specific molecular weight of the product found on the vial label and the Certificate of Analysis (available online).

Dilution Calculator

Determine the dilution needed to prepare a stock solution.

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Reconstitution Calculator

The reconstitution calculator enables you to quickly determine the volume of a reagent needed to reconstitute your vial. Just enter the mass of the reagent and the target concentration, and the calculator will do the rest.

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