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Vinculin Mouse mAb - Primary antibody, specific to Vinculin, IgG1

COA COA
    Application:
  • Flow Cytometry
  • IF/ICC
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse,Rat
  • Isotype: Mouse IgG1
  • Clone Number: AB01/1F7
  • Conjugation: Unconjugated
In stock
Item Number
Ab169192
Grouped product items
SKU Size
Availability
 Price Qty
Ab169192-10μl
10μl
≥10
$66.90
Ab169192-50μl
50μl
≥10
$199.90
Ab169192-100μl
100μl
≥10
$299.90
Ab169192-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,499.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (AB01/1F7); Mouse anti Vinculin Antibody; WB, Flow, ICC/IF, ELISA; Unconjugated

View related series
Click to view target information: VCL
Accession#:P18206 (5) Flow Cytometry (1158) Gene ID:7414 (5) VCL (5) Vinculin (4)

Basic Description

Product Name Vinculin Mouse mAb - Primary antibody, specific to Vinculin, IgG1
Synonyms CMD1W antibody | CMH15 antibody | Epididymis luminal protein 114 antibody | HEL114 antibody | Metavinculin antibody | MV antibody | MVCL antibody | OTTHUMP00000019861 antibody | OTTHUMP00000019862 antibody | VCL antibody | VINC antibody | VINC_HUMAN antib
Specifications & Purity Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA
Host species Mouse
Specificity Vinculin
Immunogen Recombinant full length protein corresponding to Human Vinculin
Positive Control WB: HeLa, HEK293, HepG2, A431, MCF-7, HUVEC, SK-N-SH, NIH/3T3, C6 whole cell lysates, Mouse lung lysates Flow: HeLa cells ICC/IF: HeLa cells
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Application:
WB: 1/1,000-1/200,000 ; Predicted molecular weight: 124 kDa
Flow: 0.04 μg/mL - 2.5 μg/mL
ICC/IF: 5.0 μg/mL

Product Properties

Isotype IgG1
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

VCL Tbio Vinculin (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Vinculin Mouse mAb (Ab169192) - Western Blot
All lanes: Vinculin Antibody (Ab169192) at 0.5 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 124.0 kDa
Observed band size: 125.8 kDa
Exposure time: 6.879 s 

Vinculin Mouse mAb (Ab169192) - Western Blot
All lanes: Vinculin Antibody (Ab169192) at 0.5 μg/mL
Samples: Lysate at 20 µg
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 124.0 kDa
Observed band size: 122.0 kDa
Exposure time: 40.1 s 

Vinculin Mouse mAb (Ab169192) - Western Blot
All lanes: Vinculin Antibody (Ab169192) at 0.5 μg/mL
Samples: Lysate at 20 µg
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 124.0 kDa
Observed band size: 122.5 kDa
Exposure time: 0.1 s 

Vinculin Mouse mAb (Ab169192) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Vinculin Mouse mAb (Ab169192) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

Vinculin Mouse mAb (Ab169192) - ICC/IF
IF analysis of Vinculin (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Vinculin Mouse mAb (Ab169192) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
Flow Cytometry

0.04 μg/mL - 2.5 μg/mL
IF/ICC

5.0 μg/mL
WB

1/1,000-1/200,000 ; Predicted molecular weight: 124 kDa

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

8 results found

Lot Number Certificate Type Date Item
ZJ24F0102566 Certificate of Analysis Jan 24, 2024 Ab169192
ZJ24F0102560 Certificate of Analysis Jan 24, 2024 Ab169192
ZJ24F0102559 Certificate of Analysis Jan 24, 2024 Ab169192
ZJ24F0102558 Certificate of Analysis Jan 24, 2024 Ab169192
ZJ24R0100222 Certificate of Analysis Jan 18, 2024 Ab169192
ZJ23F1101843 Certificate of Analysis Nov 29, 2023 Ab169192
ZJ23F1101844 Certificate of Analysis Nov 29, 2023 Ab169192
ZJ23R1100160 Certificate of Analysis Nov 22, 2023 Ab169192

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