Grouped product items

SKU

Size

Availability

Price

Qty

Ab191788-10μg

10μg

$49.90

Ab191788-25μg

25μg

$89.90

Ab191788-100μg

100μg

$209.90

Basic Description

Product Name	SIRP alpha/CD172a Rat mAb (Biotin) - Primary antibody, specific to SIRP alpha/CD172a, Rat IgG1
Synonyms	Bit \| SIRP \| SHP-1 \| Ptpns1 \| Idd13.2 \| BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs \| Brain Ig-like molecule with tyrosine-based activation motifs \| CD172 antigen-like family member A \| CD172a antigen \| CD172a \| Inhibitory
Specifications & Purity	ExactAb™, Validated, 0.5 mg/mL
Biochemical and Physiological Mechanisms	Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial
Host species	Rat
Specificity	SIRP alpha/CD172a
Immunogen	Mouse brain membrane protein
Positive Control	Flow: C57BL/6 mouse bone marrow cells.
Conjugation	Biotin
Grade	ExactAb™, Validated
Product Description	Rat anti Mouse SIRP alpha/CD172a Antibody, Monoclonal (P84), could be used for in vivo SIRPα blocking, in vitro SIRPα blocking, WB, IP, Flow and so on.

Product Properties

Isotype	Rat IgG1
Light Chain Type	kappa
Purification Method	Protein A purified
Concentration	0.5 mg/mL
Storage Temp	Store at 2-8°C,Protected from light,Do not freeze
Shipped In	Wet ice
Stability And Storage	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

SIRPA Tbio Tyrosine-protein phosphatase non-receptor type substrate 1 (0 Activities)

Discover Target: SIRPA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Flow Cytometry: SIRP alpha/CD172a Rat mAb (Biotin) (Ab191788)

SIRP alpha/CD172a Rat mAb (Biotin) (Ab191788) - Flow Cytometry
Flow cytometry analysis of SIRP alpha/CD172a expression by C57BL/6 mouse bone marrow cells (right panel) compared to an isotype control (left panel). Cells were stained with SIRP alpha/CD172a Rat mAb (Biotin) (Ab191788) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG Isotype Control Antibody (Biotin) (Ab180310).

Flow Cytometry: SIRP alpha/CD172a Rat mAb (Biotin) (Ab191788)

SIRP alpha/CD172a Rat mAb (Biotin) (Ab191788) - Flow Cytometry
Flow cytometry analysis of SIRP alpha/CD172a expression by C57BL/6 mouse bone marrow cells (red) compared to an isotype control (blue). Cells were stained with SIRP alpha/CD172a Rat mAb (Biotin) (Ab191788) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application	Dilution info
Flow Cytometry	≤0.3 μg/test, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Alternative Products

Documents & Articles

Flow Cytometry Using Methanol Permeabilization

Categories: Protocols

Tags:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

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SIRP alpha/CD172a Rat mAb (Biotin) - Primary antibody, specific to SIRP alpha/CD172a, Rat IgG1

Basic Description

Product Properties

Associated Targets(Human)

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Alternative Products

Documents & Articles

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews

SIRP alpha/CD172a Rat mAb (Biotin) - Primary antibody, specific to SIRP alpha/CD172a, Rat IgG1

Basic Description

Product Properties

Associated Targets(Human)

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Related Products

Alternative Products

Documents & Articles

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews