Grouped product items
SKU	Size	Availability	Price
Ab210603-10μg	10μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$69.90
Ab210603-50μg	50μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$199.90
Ab210603-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$299.90
Ab210603-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$1,999.90

Recombinant (P06A32); Mouse anti Flag tag Antibody; WB, Flow Cytometry, IF/ICC, IP, ELISA; Unconjugated

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DYKDDDDK (13) Flag (13) Flow Cytometry (1158)

Basic Description

Product Name	Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody - Primary antibody, specific to DYKDDDDK epitope tag
Synonyms	DDDDK epitope tag antibody \| DDDK antibody \| ddk antibody \| DYKDDDDK (also known as the FLAG® tag sequence) antibody \| DYKDDDDK antibody \| DYKDDDDK epitope tag antibody \| DYKDDDDK tag antibody \| ECS epitope tag antibody \| ECS tag antibody \| Enterokinase C
Specifications & Purity	Animal Free, Carrier Free, Recombinant, High Performance, Azide Free, Validated, ExactAb™, ≥95%(SDS-PAGE), 1.0 mg/mL
Biochemical and Physiological Mechanisms	Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties. DYKDDDDK ta
Host species	Mouse
Specificity	DYKDDDDK epitope tag
Immunogen	Synthetic DYKDDDDK peptide
Positive Control	WB: DYKDDDDK tag transfected HEK293, whole cell lysate Flow Cytometry: HEK293 cells transfected with Flag tag IF/ICC: HEK293 cells transfected with Flag and mCherry tags, HEK293 cells transfected with Flag and eGFP tags IP: HEK293 cells transfected wit
Conjugation	Unconjugated
Grade	Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, Recombinant, Validated
Product Description	Recombinant DYKDDDDK Antibody, clone P06A32, could be used for WB, Flow Cytometry, IF/ICC, ELISA and so on.

Product Properties

Light Chain Type	kappa
SDS-PAGE	150.0 KDa
Purification Method	Protein A purified
Concentration	1.0 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Images

Western Blot: Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab210603)

Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab210603) - Western Blot
All lanes: Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab210603) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 115 kDa
Observed band size: 64, 115 kDa
Exposure time: 73.3 s

Flow Cytometry: Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab210603)

Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab210603) - Flow Cytometry
Overlay histogram showing HEK293T cells transfected with a EGFP-DYKDDDDK tag using Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab210603) (Right panel). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG, Fcγ fragment specific (AF647) was used as the secondary antibody. The mouse IgG (Ab170221) was used as an isotype control (Left panel).

ICC/IF: Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab210603)

Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab210603) - ICC/IF
IF analysis of 293T cells transfected with a EGFP-DYKDDDDK tag (Green) using Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab210603) (Red). The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab210603) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (AF647) (Ab181952) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

ICC/IF: Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab210603)

Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab210603) - ICC/IF
IF analysis of 293T cells transfected with a mCherry-DYKDDDDK tag (Red) using Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab210603) (Green). The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab210603) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application	Dilution info
IP	1/200
IF/ICC	1/200
WB	1/1000 - 1/20000
Flow Cytometry	1/200 – 1/20000

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Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody - Primary antibody, specific to DYKDDDDK epitope tag