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alpha Tubulin Mouse mAb - Primary antibody, specific to alpha tubulin, IgG2b

COA COA
    Application:
  • Flow Cytometry
  • IF/ICC
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse,Rat
  • Isotype: Mouse IgG2b
  • Clone Number: CD03/1G2
  • Conjugation: Unconjugated
In stock
Item Number
Ab169013
Grouped product items
SKU Size
Availability
 Price Qty
Ab169013-10μl
10μl
≥10
$99.90
Ab169013-50μl
50μl
1
$199.90
Ab169013-100μl
100μl
1
$299.90
Ab169013-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,499.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (CD03/1G2); Mouse anti human alpha tubulin Antibody; WB, Flow, ICC/IF, ELISA; Unconjugated

View related series
Click to view target information: TUBA1B
Accession#:P68363 (3) alpha Tubulin (5) Flow Cytometry (1158) Gene ID:10376 (3) TUBA1B (3)

Basic Description

Product Name alpha Tubulin Mouse mAb - Primary antibody, specific to alpha tubulin, IgG2b
Synonyms Alpha tubulin ubiquitous antibody | Alpha-tubulin ubiquitous antibody | K alpha 1 antibody | TBA1B_HUMAN antibody | TUBA1B antibody | Tubulin alpha 1B antibody | Tubulin alpha 1B chain antibody | Tubulin alpha ubiquitous antibody | Tubulin alpha ubiquitou
Specifications & Purity ExactAb™, Validated, Carrier Free, Azide Free, High performance, 0.5 mg/mL
Host species Mouse
Specificity alpha tubulin
Immunogen Recombinant human alpha tubulin
Positive Control WB: HeLa, MCF-7, Jurkat, HepG2, A549, Raji, HEK293, NIH3T3 and C6 whole cell lysates Flow: HeLa cells ICC/IF: HeLa cells
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Application

WB: 1/1,000-1/20,000; Predicted molecular weight: 50 kDa

Flow: 0.002 μg/mL - 0.5 μg/mL

ICC/IF: 0.5 μg/mL

Product Properties

Isotype IgG2b
Light Chain Type kappa
SDS-PAGE 185 kDa
Purification Method Protein A purified
Shape Liquid
Concentration 0.5 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Associated Targets(Human)

TUBA1B Tchem Tubulin alpha-1B chain (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

alpha Tubulin Mouse mAb (Ab169013) - Western Blot
All lanes: alpha Tubulin Antibody (Ab169013) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 50.0 kDa
Observed band size: 50.0 kDa
Exposure time: 60.0 s 

alpha Tubulin Mouse mAb (Ab169013) - Flow Cytometry
Overlay histogram showing HeLa cells stained with alpha Tubulin Mouse mAb (Ab169013) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170217) was used as an isotype control (Blue).

alpha Tubulin Mouse mAb (Ab169013) - ICC/IF
IF analysis of alpha Tubulin (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with alpha Tubulin Mouse mAb (Ab169013) at 0.5 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
IF/ICC

0.5 μg/mL

Flow Cytometry

0.002 μg/mL - 0.5 μg/mL
WB

1/1,000-1/20,000; Predicted molecular weight: 50 kDa

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ24F0102673 Certificate of Analysis Feb 01, 2024 Ab169013
ZJ24F0102672 Certificate of Analysis Feb 01, 2024 Ab169013
ZJ24F0102671 Certificate of Analysis Feb 01, 2024 Ab169013

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