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Recombinant Human TNF RII/TNFRSF1B Protein, >95%(SDS-PAGE), high purityused for Cell culture, Activity Assays, ELISA, SDS-PAGE various applications

  • Expression System: E. coli
  • Accession #: P20333
  • Protein Tag: N-His & GST
  • Bioactivity: The binding activity of TNFRSF1B and TNFa, the EC50 was 0.0470~0.085 μg/ml.
  • Endotoxin Concentration: <1.0 EU/mg
  • Application: Cell culture, Activity Assays, ELISA, SDS-PAGE
In stock
Item Number
rp152522
Grouped product items
SKU Size
Availability
Price Qty
rp152522-5μg
5μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$59.90
rp152522-20μg
20μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$139.90
rp152522-100μg
100μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$449.90
rp152522-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,569.90

Carrier Free, >95%(SDS-PAGE), Active, E.coli, His&GST, 298-441aa

Basic Description

Product Name Recombinant Human TNF RII/TNFRSF1B Protein, >95%(SDS-PAGE), high purityused for Cell culture, Activity Assays, ELISA, SDS-PAGE various applications
Synonyms CD120b antigen | CD120b | Etanercept | p75 TNF receptor | p75TBPII | p75TNFR | soluble TNFR1B variant 1 | TNF RII | TNFBRp80 TNF-alpha receptor | TNF-R2 | TNFR2TNFR1B | TNF-R75 | TNFR80 | TNFRII | TNF-RII | TNF-R-II | TNFR-II | TNFRSF1B | tumor necrosis f
Grade ActiBioPure™, Azide Free, Bioactive, Carrier Free, Low Endotoxin
Product Description

Purity

>95% SDS-PAGE.


Function

Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.


Specifications & Purity ActiBioPure™, Bioactive, Carrier Free, Low Endotoxin, Azide Free, ≥95%(SDS-PAGE)
Purity >95%(SDS-PAGE)
Bioactivity The binding activity of TNFRSF1B and TNFa, the EC50 was 0.0470~0.085 μg/ml.
Endotoxin Concentration <1.0 EU/mg
Expression System E. coli
Species Human
Amino Acids 298-441 aa
Sequence MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHMDSPDLGTGGGSGIEGRGSMGYRGSEFAKVPHLPADKARGTQGPEQQHLLITAPSSSSSSLESSASALDRRAPTRNQPQAPGVEASGAGEARASTGSSDSSPGGHGTQVNVTCIVNVCSSSDHSSQCSSQASSTMGDTDSSPSESPKDEQVPFSKEECAFRSQLETPETLL
Protein Tag N-His & GST
Protein Length Protein fragment
Accession # P20333
Source Recombinant
Predicted molecular weight 44.8 kDa

Storage and Shipping

Application Cell culture, Activity Assays, ELISA, SDS-PAGE
Shape Lyophilized
Reconstitution Reconstitute in ddH2O to a concentration ≤0.1mg/mL. Do not vortex.
Storage Temp Store at -80°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Avoid repeated freeze/thaw cycles. Store at 2-8℃ for one month. Aliquot and store at -80℃ for 12 months.

Images

Recombinant Human TNF RII/TNFRSF1B Protein (rp152522)-Protein Bioactivity
Measured by its binding ability in a functional ELISA.Tumor Necrosis Factor Alpha (TNF-α) has been identified as an interactor of TNFRSF1B, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TNFRSF1B and recombinant human TNF-α. 

Recombinant Human TNF RII/TNFRSF1B Protein (rp152522)-SDS-PAGE
3μg/lane of Recombinant Human VCAM1 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 51.3 kDa under reducing conditions and 49 kDa under non-reducing conditions.

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ23F0600460 Certificate of Analysis Jun 30, 2023 rp152522
ZJ23F0600461 Certificate of Analysis Jun 30, 2023 rp152522
ZJ23F0600462 Certificate of Analysis Jun 30, 2023 rp152522

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