Determine the necessary mass, volume, or concentration for preparing a solution.
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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
rp152506-10μg
|
10μg |
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
|
$79.90
|
|
|
rp152506-50μg
|
50μg |
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
|
$189.90
|
|
|
rp152506-100μg
|
100μg |
1
|
$289.90
|
|
|
rp152506-1mg
|
1mg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$1,999.90
|
|
GMP, ≥95% (SDS-PAGE), Active, E. coli, No tag, 77-233 aa
| Product Name | Recombinant Human TNF-alpha Protein, ≥95% (SDS-PAGE), high purity |
|---|---|
| Synonyms | APC1 protein | Cachectin | Cachetin | DIF | TNF | TNF, monocyte-derived | TNFA | TNF-A | TNFalpha | TNF-alpha | TNF-alphacachectin | TNFATNF, macrophage-derived | TNFG1F | TNFSF1A | TNFSF2 | TNFSF2TNF superfamily, member 2 | tumor necrosis factor (TNF sup |
| Grade | ActiBioPure™, Animal Free, Bioactive, Carrier Free, GMP, High Performance |
| Product Description |
|
| Specifications & Purity | Animal Free, Carrier Free, GMP, Bioactive, ActiBioPure™, High Performance, ≥95%(SDS-PAGE), See COA |
| Biochemical and Physiological Mechanisms | Tumor necrosis factor-α (TNFα) is a kind of cytokine mainly produced by macrophages and monocytes, which can participate in the immune response and inflammatory response of the body. The increase of TNFα value is closely related to sepsis, malignant tumor |
| Purity | ≥95% (SDS-PAGE) |
| Bioactivity | Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D (Catalog # A113142). The ED₅₀ for this effect is typically <0.05 ng/mL. |
| Endotoxin Concentration | <0.1 EU/μg |
| Expression System | E.coli |
| Species | Human |
| Amino Acids | 77-233 aa |
| Sequence | VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVV PSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSP CQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQV YFGIIAL |
| Protein Tag | No tag |
| N-terminal Sequence | Val |
| Protein Length | Full length protein |
| Accession # | P01375 |
| Source | Recombinant |
| Predicted molecular weight | 17.4 kD |
| Shape | liquid |
|---|---|
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -80 ℃ for 24 months. Store at -20 ℃ for 12 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
Recombinant Human TNF-alpha Protein (rp152506)-Protein Bioactivity
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D (Catalog # A113142). The EC₅₀ for this effect is typically <0.05ng/mL.
Recombinant Human TNF-alpha Protein (rp152506)-SDS-PAGE
2μg/lane of Recombinant Human TNF-alpha was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing a single band at 16 kDa.
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Apr 25, 2024 | rp152506 | |
| Certificate of Analysis | Jan 25, 2024 | rp152506 | |
| Certificate of Analysis | Jan 25, 2024 | rp152506 | |
| Certificate of Analysis | Jul 14, 2023 | rp152506 | |
| Certificate of Analysis | Mar 25, 2023 | rp152506 |
Starting at $99.90
Starting at $99.90