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Recombinant Human IL-2 Protein, ≥95% (SDS-PAGE, SEC-HPLC), high purity

Grade & Purity:

≥95%(SDS-PAGE)

Expression System: CHO-K1
Accession #: P60568
Protein Tag: His
Bioactivity: 1、Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is typically less than 2.5 ng/mL. 2、Measured in a cell proliferation assay using mouse splenocytes. The ED50 for this effect is typically less than 30.0 ng/mL.
Endotoxin Concentration: <1.0 EU/μg

In stock

Item Number

rp147476

Grouped product items
SKU	Size	Availability	Price
rp147476-10μg	10μg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$59.90
rp147476-50μg	50μg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$139.90
rp147476-100μg	100μg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$229.90
rp147476-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$699.90

GMP, ≥95% (SDS-PAGE, SEC-HPLC), Active, CHO-K1, His, 21-153 aa

View related series

Click to view protein information: IL2

Accession#:P60568 (2) Gene ID:3558 (2) IL2 (5)

Basic Description

Product Name	Recombinant Human IL-2 Protein, ≥95% (SDS-PAGE, SEC-HPLC), high purity
Synonyms	IL-2 \| T-cell growth factor \| TCGF
Grade	ActiBioPure™, Azide Free, Bioactive, Carrier Free, GMP, High Performance
Product Description	Purity ≥95% (SDS-PAGE, SEC-HPLC) Additional sequence information 133 amino acids Function Interleukin-2, or interleukin-2, is also known as T cell growth factor (TCRF). A wide range of bioactive cytokines produced primarily by activated CD4+T cells and CD8+T cells. It is a growth factor of all T cell subsets, which can make T cells survive in vitro for a long time and promote the proliferation of activated B cells. Therefore, it is an important factor in the regulation of immune response, and also involved in antibody response, hematopoietic and tumor surveillance. Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. This product is a recombinant protein expressed by eukaryotic cells. The protein gene was inserted into stable expression vector and transformed into CHO-K1 cells. After cloning, screening and quality verification, the selected stable expression strain was prepared by fermentation of the medium determined by chemical composition (without any animal source components). This product is a sterile lyophilized powder, made from a protein solution containing 10mM phosphate with a pH of 7.2 filtered by 0.2μm.
Specifications & Purity	ActiBioPure™, Bioactive, GMP, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
Purity	≥95% (SDS-PAGE, SEC-HPLC)
Bioactivity	1、Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is typically less than 2.5 ng/mL. 2、Measured in a cell proliferation assay using mouse splenocytes. The ED50 for this effect is typically less than 30.0 ng/mL.
Endotoxin Concentration	<1.0 EU/μg
Expression System	CHO-K1
Species	Human
Amino Acids	21-153 aa
Sequence	APTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT
Protein Tag	His
Protein Length	Full length protein
Accession #	P60568
Source	Recombinant
Predicted molecular weight	15.4 kDa
SDS-PAGE	13.0 kDa, under reducing conditions; 13.0 kDa, under non-reducing conditions.

Storage and Shipping

Shape	Lyophilized
Reconstitution	It is recommended to dissolve the lyophilized rhIL-2 in water for injection or sterilized ultra-pure water with a concentration of not less than 100μg/ml for further dilution to working concentration. Avoid repeated freeze-thaw as much as possible. Redissolved cytokines can be stably stored at 4℃ for 7-10 days. Please store at -20℃ after subassembly for short-term storage.
Storage Temp	Store at -20°C
Shipped In	Ice chest + Ice pads
Stability And Storage	Shipped at 4°C. Store at -20°C. Lyophilized with no additives. This product is an active protein and may elicit a biological response in vivo, handle with caution.

Images

Protein Bioactivity: Recombinant Human IL-2 Protein (rp147476)

Recombinant Human IL-2 Protein (rp147476)-Protein Bioactivity
Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED₅₀ for this effect is typically <2.5ng/mL.

SDS-PAGE: Recombinant Human IL-2 Protein (rp147476)

Recombinant Human IL-2 Protein (rp147476) -SDS-PAGE
3μg/lane of Recombinant Human IL-2 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 13.0 kDa.

Protein Bioactivity: Recombinant Human IL-2 Protein (rp147476)

Recombinant Human IL-2 Protein (rp147476) - Protein Bioactivity
Measured in a cell proliferation assay using mouse splenocytes. The ED50 for this effect is typically less than 30.0 ng/mL.

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Analytical Chart:

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3 results found

Lot Number	Certificate Type	Date	Item
ZJ23F0600484	Certificate of Analysis	Jul 03, 2023	rp147476
ZJ23F0600486	Certificate of Analysis	Jul 03, 2023	rp147476
ZJ23F0600485	Certificate of Analysis	Jul 03, 2023	rp147476

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