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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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rp145939-50μg
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50μg |
5
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$189.90
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rp145939-100μg
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100μg |
1
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$229.90
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rp145939-1mg
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1mg |
1
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$799.90
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rp145939-500μg
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500μg |
3
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$479.90
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GMP, ≥95% SDS-PAGE, Active, E. coli , No tag, 134-288 aa
| Product Name | Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein, >95% SDS-PAGE, high purity |
|---|---|
| Synonyms | Basic fibroblast growth factor Basic fibroblast growth factor bFGF BFGF FGF 2 FGF B FGF-2 Fgf2 FGF2 basic FGF2_HUMAN FGFB Fibroblast growth factor 2 Fibroblast growth factor 2 (basic) Fibroblast growth factor, basic HBGF 2 HBGF-2 HBGF2 HBGH 2 HBGH2 Hepari |
| Grade | ActiBioPure™, Animal Free, Bioactive, Carrier Free, GMP, High Performance |
| Product Description |
Recombinant human basic fibroblast growth factor (also known as basic FGF, bFGF, FGF2, FGF-beta, or heparin-binding growth factor), is a biologically active protein suitable for cell culture applications. bFGF regulates diverse processes such as cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation, and wound recovery. bFGF can be used in studies of angiogenesis, fibroblast mitosis, axonal outgrowth in PC-12 cells, receptor binding, and tyrosine phosphorylation. This strain is expressed in recombinant Escherichia coli, and after multi-step separation and purification, it is dissolved in 10mM PBS, 0.15 M NaCl (pH7.2) solution, filtered through a 0.22 μm filter membrane, and then freeze-dried to make a lyophilized powder.
|
| Specifications & Purity | GMP, Bioactive, ActiBioPure™, Animal Free, Carrier Free, High Performance, ≥95%(SDS-PAGE) |
| Purity | >95% SDS-PAGE |
| Bioactivity | Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <0.9 ng/mL. |
| Endotoxin Concentration | <0.2 EU/μg |
| Expression System | E.coli |
| Species | Human |
| Amino Acids | 134-288 aa |
| Sequence | MAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS |
| Protein Tag | No tag |
| Protein Length | Full length protein |
| Accession # | P09038 |
| Source | Recombinant |
| Predicted molecular weight | 17 kDa |
| Shape | Lyophilized |
|---|---|
| Reconstitution | It is recommended to briefly centrifuge before opening the cap to make the powder sink to the bottom of the tube, then dissolve it in sterilized deionized water to prepare a 100μg/ml stock solution, which can be stored stably for one week at 2°C ~ 8°C. When diluting to a lower concentration (not lower than 10μg/ml), 0.1% tissue culture grade BSA or HSA needs to be added. If extended storage time is required, it is recommended to freeze at -80°C or -20°C, repeated freezing and thawing is not recommended. |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 2-8℃ short term (4 weeks). Store at -20℃ long term (24 months). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| CAS | 62031-54-3 |
Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein (rp145939)-SDS-PAGE
2μg/lane of Recombinant Human FGF2 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 15.6 kDa.
Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein (rp145939)-Protein Bioactivity
Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <0.9ng/mL.
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Apr 07, 2023 | rp145939 | |
| Certificate of Analysis | Apr 06, 2023 | rp145939 | |
| Certificate of Analysis | Apr 06, 2023 | rp145939 | |
| Certificate of Analysis | Apr 06, 2023 | rp145939 |
Starting at $59.90
Starting at $99.90