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Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein, >95% SDS-PAGE, high purity

  • Expression System: E.coli
  • Accession #: P09038
  • Protein Tag: No tag
  • Bioactivity: Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <0.9 ng/mL.
  • Endotoxin Concentration: <0.2 EU/μg
In stock
Item Number
rp145939
Grouped product items
SKU Size
Availability
Price Qty
rp145939-50μg
50μg
5
$189.90
rp145939-100μg
100μg
1
$229.90
rp145939-1mg
1mg
1
$799.90
rp145939-500μg
500μg
3
$479.90

GMP, ≥95% SDS-PAGE, Active, E. coli , No tag, 134-288 aa

Basic Description

Product Name Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein, >95% SDS-PAGE, high purity
Synonyms Basic fibroblast growth factor Basic fibroblast growth factor bFGF BFGF FGF 2 FGF B FGF-2 Fgf2 FGF2 basic FGF2_HUMAN FGFB Fibroblast growth factor 2 Fibroblast growth factor 2 (basic) Fibroblast growth factor, basic HBGF 2 HBGF-2 HBGF2 HBGH 2 HBGH2 Hepari
Grade ActiBioPure™, Animal Free, Bioactive, Carrier Free, GMP, High Performance
Product Description

Recombinant human basic fibroblast growth factor (also known as basic FGF, bFGF, FGF2, FGF-beta, or heparin-binding growth factor), is a biologically active protein suitable for cell culture applications. bFGF regulates diverse processes such as cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation, and wound recovery. bFGF can be used in studies of angiogenesis, fibroblast mitosis, axonal outgrowth in PC-12 cells, receptor binding, and tyrosine phosphorylation. This strain is expressed in recombinant Escherichia coli, and after multi-step separation and purification, it is dissolved in 10mM PBS, 0.15 M NaCl (pH7.2) solution, filtered through a 0.22 μm filter membrane, and then freeze-dried to make a lyophilized powder.

Specifications & Purity GMP, Bioactive, ActiBioPure™, Animal Free, Carrier Free, High Performance, ≥95%(SDS-PAGE)
Purity >95% SDS-PAGE
Bioactivity Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <0.9 ng/mL.
Endotoxin Concentration <0.2 EU/μg
Expression System E.coli
Species Human
Amino Acids 134-288 aa
Sequence MAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS
Protein Tag No tag
Protein Length Full length protein
Accession # P09038
Source Recombinant
Predicted molecular weight 17 kDa

Storage and Shipping

Shape Lyophilized
Reconstitution It is recommended to briefly centrifuge before opening the cap to make the powder sink to the bottom of the tube, then dissolve it in sterilized deionized water to prepare a 100μg/ml stock solution, which can be stored stably for one week at 2°C ~ 8°C. When diluting to a lower concentration (not lower than 10μg/ml), 0.1% tissue culture grade BSA or HSA needs to be added. If extended storage time is required, it is recommended to freeze at -80°C or -20°C, repeated freezing and thawing is not recommended.
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 2-8℃ short term (4 weeks). Store at -20℃ long term (24 months). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.
CAS 62031-54-3

Images

Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein (rp145939)-SDS-PAGE
2μg/lane of Recombinant Human FGF2 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 15.6 kDa.

Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein (rp145939)-Protein Bioactivity
Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <0.9ng/mL.

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot Number Certificate Type Date Item
ZJ23F0400109 Certificate of Analysis Apr 07, 2023 rp145939
ZJ23F0400108 Certificate of Analysis Apr 06, 2023 rp145939
ZJ23F0400107 Certificate of Analysis Apr 06, 2023 rp145939
ZJ23F0400097 Certificate of Analysis Apr 06, 2023 rp145939

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