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Recombinant F4/80 Antibody (Biotin) - Primary antibody, specific to F4/80

    Application:
  • Flow Cytometry
  • Host species: Rat
  • Reactive species: Mouse
  • Isotype: Rat IgG2a
  • Clone Number: P20A44
  • Conjugation: Biotin
In stock
Item Number
Ab213900
Grouped product items
SKU Size
Availability
Price Qty
Ab213900-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$14.90
Ab213900-25μg
25μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$28.90
Ab213900-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$81.90

Recombinant (P20A44); Rat anti Mouse F4/80 Antibody (Biotin); Flow Cytometry

Basic Description

Product Name Recombinant F4/80 Antibody (Biotin) - Primary antibody, specific to F4/80
Synonyms ADGRE1 antibody | Adhesion G protein coupled receptor E1 antibody | Adhesion G protein-coupled receptor E1 antibody | AGRE1_HUMAN antibody | Cell surface glycoprotein EMR1 antibody | Cell surface glycoprotein F4/80 antibody | DD7A5 7 antibody | Egf like m
Specifications & Purity ExactAb™, Validated, 0.5 mg/mL
Biochemical and Physiological Mechanisms Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
Host species Rat
Specificity F4/80
Immunogen Murine macrophages
Positive Control Flow Cytometry: C57BL/6 mouse bone marrow cells
Conjugation Biotin
Grade ExactAb™, Validated
Product Description

Recombinant F4/80 Antibody, clone P20A44, could be used for Flow Cytometry.

Product Properties

Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Concentration 0.5 mg/mL
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

ADGRE1 Tbio Adhesion G protein-coupled receptor E1 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Recombinant F4/80 Antibody (Biotin) (Ab213900) - Flow Cytometry
Two-color flow cytometric analysis of F4/80 expression on C57BL/6 mouse bone marrow cells. C57BL/6 mouse bone marrow cells were stained with CD11b Rat mAb (APC) (Ab178881) and either Rat IgG (Biotin) (Ab180310; Left panel) or Recombinant F4/80 Antibody (Biotin) (Ab213900; Right panel) at 0.2μg/test. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark.

Recombinant F4/80 Antibody (Biotin) (Ab213900) - Flow Cytometry
Flow cytometry analysis of F4/80 expression by C57BL/6 mouse bone marrow cells (red) compared to an isotype control (blue). Cells were stained with Recombinant F4/80 Antibody (Biotin) (Ab213900) at 0.1 μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue – Rat IgG (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

≤0.3μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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