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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab213900-10μg
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10μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$14.90
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Ab213900-25μg
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25μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$28.90
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Ab213900-100μg
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100μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$81.90
|
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Recombinant (P20A44); Rat anti Mouse F4/80 Antibody (Biotin); Flow Cytometry
| Product Name | Recombinant F4/80 Antibody (Biotin) - Primary antibody, specific to F4/80 |
|---|---|
| Synonyms | ADGRE1 antibody | Adhesion G protein coupled receptor E1 antibody | Adhesion G protein-coupled receptor E1 antibody | AGRE1_HUMAN antibody | Cell surface glycoprotein EMR1 antibody | Cell surface glycoprotein F4/80 antibody | DD7A5 7 antibody | Egf like m |
| Specifications & Purity | ExactAb™, Validated, 0.5 mg/mL |
| Biochemical and Physiological Mechanisms | Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. |
| Host species | Rat |
| Specificity | F4/80 |
| Immunogen | Murine macrophages |
| Positive Control | Flow Cytometry: C57BL/6 mouse bone marrow cells |
| Conjugation | Biotin |
| Grade | ExactAb™, Validated |
| Product Description |
Recombinant F4/80 Antibody, clone P20A44, could be used for Flow Cytometry. |
| Light Chain Type | kappa |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at 2-8°C,Protected from light,Do not freeze |
| Shipped In | Wet ice |
| Stability And Storage | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Recombinant F4/80 Antibody (Biotin) (Ab213900) - Flow Cytometry
Two-color flow cytometric analysis of F4/80 expression on C57BL/6 mouse bone marrow cells. C57BL/6 mouse bone marrow cells were stained with CD11b Rat mAb (APC) (Ab178881) and either Rat IgG (Biotin) (Ab180310; Left panel) or Recombinant F4/80 Antibody (Biotin) (Ab213900; Right panel) at 0.2μg/test. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark.
Recombinant F4/80 Antibody (Biotin) (Ab213900) - Flow Cytometry
Flow cytometry analysis of F4/80 expression by C57BL/6 mouse bone marrow cells (red) compared to an isotype control (blue). Cells were stained with Recombinant F4/80 Antibody (Biotin) (Ab213900) at 0.1 μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue – Rat IgG (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.
| Application | Dilution info |
|---|---|
| Flow Cytometry | ≤0.3μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume. |
Starting at $69.90
Starting at $16.90
Starting at $14.90
Starting at $69.90