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RANKL/CD254 Rat mAb - Primary antibody, specific to RANKL/CD254, Rat IgG2a

    Application:
  • Blocking
  • Flow Cytometry
  • Functional Assay
  • IF/ICC
  • IHC
  • IP
  • IP-MS
  • WB
  • Host species: Rat
  • Reactive species: Mouse
  • Isotype: Rat IgG2a
  • Clone Number: IK22/5
  • Conjugation: Unconjugated
In stock
Item Number
Ab124551
Grouped product items
SKU Size
Availability
Price Qty
Ab124551-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$29.90
Ab124551-50μg
50μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab124551-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$99.90
Ab124551-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$199.90

mAb (IK22/5); Rat anti Mouse RANKL/CD254 Antibody; Flow, IP, WB, IF, ICC, IHC, Blocking; Unconjugated

Basic Description

Product Name RANKL/CD254 Rat mAb - Primary antibody, specific to RANKL/CD254, Rat IgG2a
Synonyms Ly109l | Trance | CD254 antigen | CD254 | ODF | OPGL | OPGLOPTB2 | Osteoclast differentiation factor | Osteoprotegerin ligand | RANK L | RANKL | RANKLreceptor activator of nuclear factor kappa B ligand | Receptor activator of nuclear factor kappa-B ligand
Specifications & Purity Carrier Free, Low Endotoxin, Azide Free, Validated, ≥95%(SDS-PAGE&HPLC), See COA
Host species Rat
Specificity RANKL/CD254
Immunogen Purified Recombinant Mouse RANKL (NSO-derived)
Conjugation Unconjugated
Grade Azide Free, Carrier Free, Low Endotoxin, Validated
Product Description

Rat anti Mouse RANKL/CD254 Antibody, Monoclonal (IK22/5), could be used for Flow, IP, WB, IF, ICC, IHC, Blocking and so on.
Application
Flow: ≤ 0.25µg/100w cells
IP: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent concentration.
IHC: Use at an assay dependent concentration.
Blocking: Use at an assay dependent concentration.
Protein Function
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.

Product Properties

Isotype Rat IgG2a
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

TNFSF11 Tclin Tumor necrosis factor ligand superfamily member 11 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

RANKL/CD254 Rat mAb (Ab124551) - ELISA
Immobilized Recombinant Mouse TRANCE/RANKL/TNFSF11 protein (rp154577) at 1.0 μg/mL can bind RANKL/CD254 Rat mAb (Ab124551) with the EC50 of 0.977 ng/mL.

Application

Application Dilution info
Flow Cytometry

≤0.25μg/100w cells

WB

Use at an assay dependent concentration.

Blocking

Use at an assay dependent concentration.

IHC

Use at an assay dependent concentration.

IP-MS

Use at an assay dependent concentration.

IF/ICC

Use at an assay dependent concentration.

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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