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PD1/CD279 Rat mAb - Primary antibody, specific to PD1/CD279, Rat IgG2a

COA COA
    Application:
  • Animal Model
  • Blocking
  • Flow Cytometry
  • Functional Assay
  • IF/ICC
  • IHC
  • Neutralization
  • WB
  • Host species: Rat
  • Reactive species: Mouse
  • Isotype: Rat IgG2a
  • Clone Number: 29F.1A12
  • Conjugation: Unconjugated
In stock
Item Number
Ab120765
Grouped product items
SKU Size
Availability
 Price Qty
Ab120765-10μg
10μg
10
$29.90
Ab120765-50μg
50μg
4
$69.90
Ab120765-100μg
100μg
2
$99.90
Ab120765-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$229.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (29F.1A12); Rat anti Mouse PD1/CD279 Antibody; in vivo blocking of PD-1/PD-L signaling, in vitro PD-1 neutralization, IHC, ICC/IF, WB, Flow; Unconjugated

View related series
Click to view target information: PDCD1
Accession#: Q02242 (4) CD279 (28) Flow Cytometry (1158) Gene ID: 18566 (4) PD1 (37) PDCD1 (39)

Basic Description

Product Name PD1/CD279 Rat mAb - Primary antibody, specific to PD1/CD279, Rat IgG2a
Synonyms Pdc1 | Ly101 | CD279 antigen | CD279 | hPD-1 | PD1 | PD-1 | PD1hPD-l | PDCD1 | programmed cell death 1 | programmed cell death protein 1 | Protein PD-1 | SLEB2 | CD279 antibody | CD279 antigen antibody | hPD 1 antibody | hPD l antibody | hPD-1 antibody |
Specifications & Purity Carrier Free, Azide Free, Validated, ≥95%(SDS-PAGE), See COA
Host species Rat
Specificity PD1/CD279
Immunogen Recombinant protein of mouse PD-1-Ig fusion
Conjugation Unconjugated
Grade Azide Free, Carrier Free, Validated
Product Description

Rat anti Mouse PD1/CD279 Antibody, Monoclonal (29F.1A12), could be used for in vivo blocking of PD-1/PD-L signaling, in vitro PD-1 neutralization, IHC, ICC/IF, WB, Flow and so on.
Application:
in vivo blocking of PD-1/PD-L signaling: Use at an assay dependent concentration.
in vitro PD-1 neutralization: Use at an assay dependent concentration.
IHC: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.
Flow: Use at an assay dependent concentration.
Protein Function
Possible cell death inducer, in association with other factors.

Product Properties

Isotype Rat IgG2a
Purification Method Protein G purified
Shape Liquid
Concentration See COA
Storage Temp Store at 2-8°C,Avoid repeated freezing and thawing
Shipped In Wet ice
Stability And Storage Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Associated Targets(Human)

PDCD1 Tclin Programmed cell death protein 1 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
PDCD1 Tclin Programmed cell death protein 1 (14 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

PD1/CD279 Rat mAb (Ab120765) - Flow Cytometry
Two-color flow cytometric analysis of PD1/CD279 expression on mouse splenocytes. Flow Cytometry analysis of unstimulated (left) or 500 ng/mL Ionomycin (I139530) and 10 ng/mL PMA (P408905) co-stimulated (right) C57BL/6 mouse spleen cells were stained with CD4 Rat mAb (FITC) (Ab181110) and PD1/CD279 Rat mAb (Ab120765) at 0.5 μg/mL. Two-color flow cytometric dot plots showing the correlated expression of CD4 versus PD1/CD279 (or Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells.

Application

Application Dilution info
WB

Use at an assay dependent concentration.
IHC

Use at an assay dependent concentration.
IF/ICC

Use at an assay dependent concentration.
Flow Cytometry

Use at an assay dependent concentration.
in vivo blocking of PD-1/PD-L signaling

Use at an assay dependent concentration.
in vitro PD-1 neutralization

Use at an assay dependent concentration.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ24F0708858 Certificate of Analysis Jul 26, 2024 Ab120765
ZJ24F0708857 Certificate of Analysis Jul 26, 2024 Ab120765
ZJ24F0708856 Certificate of Analysis Jul 26, 2024 Ab120765

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