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Cloning & Protein Expression
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Protein G-MNase (pG-MNase)Species: Staphylococcus aureus Accession #: UPI000AAD0772Expression system: E. coliBioactivity: One Agarose Gel Unit is defined as the amount of enzyme required to digest 1μg of Lambda DNA in 15 minutes at 37℃, to the extent that the accumulation of low molecular DNA fragments is <400 base pairs as determined by agarose gel electrophoresis. Another Unit is Kunitz Unit. One Kunitz Unit is defined as the amount of enzyme required to release acid soluble oligonucleotides that produce an absorbance increase of O.D. 1.0 at 260nm in 30 minutes at 37 ℃ . 1000 Agarose Gel Units is approximately equal to 100 Kunitz Units.
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T4 RNA LigaseSpecies: Enterobacteria phage T4 Accession #: 2C5U_B,P00971Expression system: E. coliBioactivity: One unit is defined as the amount of enzyme required to catalyze 1nmol of 5'-[32P]-(A)12-18 to a phosphatase-indigestible cyclic product within 30 minutes at 37℃.Synonyms: rnlA
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T4 gene 32 protein (ssDNA binding protein)Species: Escherichia phage T4 Accession #: P03695Expression system: E. coliSynonyms: T4 gp32 protein | T4 phage gp32 protein；Single-stranded DNA-binding protein
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T3 RNA PolymeraseAccession #: YP_009792932.1,P07659Expression system: E. coliBioactivity: One unit of enzyme incorporates 1nmol of AMP into polynucleotide in 1 hour at 37°C under the following activity assay conditions.
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T4 RNA Ligase 2, truncatedSpecies: Enterobacteria phage T4 Accession #: P32277.1Expression system: E. coliBioactivity: 200 units is defined as the amount of enzyme required to give 80% ligation of a 31-mer RNA to the pre-adenylated end of a 17-mer DNA (Universal miRNA Cloning Linker) in a total reaction volume of 10 µl in 1 hour at 25℃.Synonyms: T4 Rnl2, truncated | Rnl2 (1-249） | RNA ligase 2 | rnlB
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T4 RNA Ligase 2, truncated KQSpecies: Enterobacteria phage T4 Accession #: P32277.1Expression system: E. coliBioactivity: 200 units is defined as the amount of enzyme required to give 80% ligation of a 31-mer RNA to the pre-adenylated end of a 17-mer DNA (Universal miRNA Cloning Linker) in a total reaction volume of 20µl in 1 hour at 25℃.Synonyms: T4 Rnl2tr KQ | T4 Rnl2tr R55K, K227Q
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Vaccinia DNA Topoisomerase ISpecies: Vaccinia virus Accession #: P68698.1Expression system: E. coliBioactivity: One unit of DNA Topoisomerase I from Vaccinia converts 1 µg of supercoiled closed circular pUC18 DNA to relaxed closed circular form in 30 min at 37°C.Synonyms: Vaccinia Virus DNA Topoisomerase I | H6R
