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T4 RNA Ligase 2, truncated KQ - Animal Free, DNase, RNase free, Protease Free, sterile, Carrier Free, Bioactive, ActiBioPure™, EnzymoPure™, ≥95%(SDS-PAGE), 200 U/µL, high purity

  • Accession #: P32277.1
  • Bioactivity: 200 units is defined as the amount of enzyme required to give 80% ligation of a 31-mer RNA to the pre-adenylated end of a 17-mer DNA (Universal miRNA Cloning Linker) in a total reaction volume of 20µl in 1 hour at 25℃.
In stock
Item Number
T750875
Grouped product items
SKU Size
Availability
Price Qty
T750875-5KU
5KU
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$348.90
T750875-4×5KU
4×5KU
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,058.90
T750875-20×5KU
20×5KU
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$3,958.90

Basic Description

Product Name T4 RNA Ligase 2, truncated KQ - Animal Free, DNase, RNase free, Protease Free, sterile, Carrier Free, Bioactive, ActiBioPure™, EnzymoPure™, ≥95%(SDS-PAGE), 200 U/µL, high purity
Synonyms T4 Rnl2tr KQ | T4 Rnl2tr R55K, K227Q
Grade ActiBioPure™, Animal Free, Bioactive, Carrier Free, DNase, RNase free, Protease Free, sterile, EnzymoPure™
Specifications & Purity Animal Free, DNase, RNase free, Protease Free, sterile, Carrier Free, Bioactive, ActiBioPure™, EnzymoPure™, ≥95%(SDS-PAGE), 200 U/µL
Biochemical and Physiological Mechanisms Repairs 3'-OH/5'-PO4 nicks in duplex RNA or RNA:DNA hybrid in which the broken 3'-OH strand is RNA (Probable) .The nick ligation reaction entails three nucleotidyl transfer steps. In the first step, the RNA ligase reacts with ATP in the absence of nucleic
Bioactivity 200 units is defined as the amount of enzyme required to give 80% ligation of a 31-mer RNA to the pre-adenylated end of a 17-mer DNA (Universal miRNA Cloning Linker) in a total reaction volume of 20µl in 1 hour at 25℃.
Accession # P32277.1

Storage and Shipping

Concentration 200 U/µL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20℃ long term (24 months). Avoid freeze/thaw cycle.
Unit definition 200 units is defined as the amount of enzyme required to give 80% ligation of a 31-mer RNA to the pre-adenylated end of a 17-mer DNA in a total reaction volume of 20μl in 1 hour at 25℃.

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

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