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SKU | Size | Availability |
Price | Qty |
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T750875-5KU
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5KU |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$348.90
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T750875-4×5KU
|
4×5KU |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$1,058.90
|
|
T750875-20×5KU
|
20×5KU |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$3,958.90
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|
Product Name | T4 RNA Ligase 2, truncated KQ - Animal Free, DNase, RNase free, Protease Free, sterile, Carrier Free, Bioactive, ActiBioPure™, EnzymoPure™, ≥95%(SDS-PAGE), 200 U/µL, high purity |
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Synonyms | T4 Rnl2tr KQ | T4 Rnl2tr R55K, K227Q |
Grade | ActiBioPure™, Animal Free, Bioactive, Carrier Free, DNase, RNase free, Protease Free, sterile, EnzymoPure™ |
Specifications & Purity | Animal Free, DNase, RNase free, Protease Free, sterile, Carrier Free, Bioactive, ActiBioPure™, EnzymoPure™, ≥95%(SDS-PAGE), 200 U/µL |
Biochemical and Physiological Mechanisms | Repairs 3'-OH/5'-PO4 nicks in duplex RNA or RNA:DNA hybrid in which the broken 3'-OH strand is RNA (Probable) .The nick ligation reaction entails three nucleotidyl transfer steps. In the first step, the RNA ligase reacts with ATP in the absence of nucleic |
Bioactivity | 200 units is defined as the amount of enzyme required to give 80% ligation of a 31-mer RNA to the pre-adenylated end of a 17-mer DNA (Universal miRNA Cloning Linker) in a total reaction volume of 20µl in 1 hour at 25℃. |
Accession # | P32277.1 |
Concentration | 200 U/µL |
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Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
Shipped In | Ice chest + Ice pads |
Stability And Storage | Store at -20℃ long term (24 months). Avoid freeze/thaw cycle. |
Unit definition | 200 units is defined as the amount of enzyme required to give 80% ligation of a 31-mer RNA to the pre-adenylated end of a 17-mer DNA in a total reaction volume of 20μl in 1 hour at 25℃. |