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Cloning & Protein Expression
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Antarctic Phosphatase (Thermosensitive)Species: Antarctic bacterium TAB5 Accession #: 2W5W_B,Q9KWY4Expression system: E. coliBioactivity: 1 unit refers to the 5 'concave end produced by restriction endonuclease digestion at 37 ℃ for 30 minutes. The enzyme amount required for dephosphorylation of µ g pUC19 vector DNA. The dephosphorylation standard refers to the self linking reaction Can inhibit>95% of DNA re circularization and detect it by transforming E. coli cells.Synonyms: Thermosensitive phosphatase
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Bst DNA Polymerase, Large FragmentSpecies: Geobacillus stearothermophilus Accession #: Q45458Expression system: E. coliBioactivity: One unit is defined as the amount of enzyme that will incorporate 10nmol of dNTP into acid insoluble material in 30 minutes at 65℃.Synonyms: (Bacillus stearothermophilus, Bst) DNA Polymerase I , Large Fragment | DNA polymerase I | polA
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Cre RecombinaseSpecies: Escherichia phage P1 Accession #: Q5QBM7Expression system: E. coliBioactivity: One unit is defined as the amount of enzyme required to achieve the maximum site-specific recombination of 250ng of control DNA in 30 minutes at 37ºC in a 50μl reaction system. The maximum recombination can be analyzed by agarose gel electrophoresis or by screening on the corresponding resistance plates after transformation of the reactions.Synonyms: Cyclization Recombination Enzyme | Type I topoisomerase | tyrosine recombinase
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Klenow Fragment, Exo-Species: E. coli Accession #: 2KFZ_A,P00582Expression system: E. coliBioactivity: One unit is the amount of enzyme required to catalyze the incorporation of 10nmol of deoxyribonucleotides (dNTPs) into a polynucleotide within 30 minutes at 37℃.Synonyms: DNA polymerase I Fragment Mutant
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Lambda ExonucleaseSynonyms: λ Exonuclease | exo | lambdap24
