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MHC class II I E kappa Mouse mAb (Biotin) - Primary antibody, specific to MHC class II I E kappa

    Application:
  • Flow Cytometry
  • Host species: Mouse
  • Reactive species: Mouse,Rat
  • Isotype: Mouse IgG2a
  • Clone Number: 14-4-4S
  • Conjugation: Biotin
In stock
Item Number
Ab210121
Grouped product items
SKU Size
Availability
Price Qty
Ab210121-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$18.90
Ab210121-25μg
25μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$37.90
Ab210121-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$99.90

mAb (14-4-4S); Mouse anti Mouse/Rat MHC class II I E kappa Antibody (Biotin); Flow Cytometry

Basic Description

Product Name MHC class II I E kappa Mouse mAb (Biotin) - Primary antibody, specific to MHC class II I E kappa
Synonyms Eb | Ia4 | H2Eb | Ia-4 | H-2Eb | H2 Ea antibody | H2-Ea antibody
Specifications & Purity ExactAb™, Validated, 0.5 mg/mL
Host species Mouse
Specificity MHC class II I E kappa
Immunogen C3H mouse skin graft and spleen cells
Positive Control Flow: rat splenocytes
Conjugation Biotin
Grade ExactAb™, Validated
Product Description

Mouse anti Mouse/Rat MHC class II I E kappa Antibody, Monoclonal (14-4-4S), could be used for Flow Cytometry.

Product Properties

Light Chain Type kappa
Purification Method Protein G purified
Concentration 0.5 mg/mL
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

HLA-DRB5 Tbio HLA class II histocompatibility antigen, DR beta 5 chain (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

MHC class II I E kappa Mouse mAb (Biotin) (Ab210121) - Flow Cytometry
Flow cytometry analysis of MHC class II I E kappa expression by rat splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with MHC class II I E kappa Mouse mAb (Biotin) (Ab210121) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark.  Left panel – Mouse IgG (Biotin) (Ab180306).

MHC class II I E kappa Mouse mAb (Biotin) (Ab210121) - Flow Cytometry
Flow cytometry analysis of MHC class II I E kappa expression by rat splenocytes cells (red) compared to an isotype control (blue). Cells were stained with MHC class II I E kappa Mouse mAb (Biotin) (Ab210121) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

≤0.3μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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