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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab210121-10μg
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10μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$18.90
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Ab210121-25μg
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25μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$37.90
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Ab210121-100μg
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100μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$99.90
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mAb (14-4-4S); Mouse anti Mouse/Rat MHC class II I E kappa Antibody (Biotin); Flow Cytometry
| Product Name | MHC class II I E kappa Mouse mAb (Biotin) - Primary antibody, specific to MHC class II I E kappa |
|---|---|
| Synonyms | Eb | Ia4 | H2Eb | Ia-4 | H-2Eb | H2 Ea antibody | H2-Ea antibody |
| Specifications & Purity | ExactAb™, Validated, 0.5 mg/mL |
| Host species | Mouse |
| Specificity | MHC class II I E kappa |
| Immunogen | C3H mouse skin graft and spleen cells |
| Positive Control | Flow: rat splenocytes |
| Conjugation | Biotin |
| Grade | ExactAb™, Validated |
| Product Description |
Mouse anti Mouse/Rat MHC class II I E kappa Antibody, Monoclonal (14-4-4S), could be used for Flow Cytometry. |
| Light Chain Type | kappa |
|---|---|
| Purification Method | Protein G purified |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at 2-8°C,Protected from light,Do not freeze |
| Shipped In | Wet ice |
| Stability And Storage | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
MHC class II I E kappa Mouse mAb (Biotin) (Ab210121) - Flow Cytometry
Flow cytometry analysis of MHC class II I E kappa expression by rat splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with MHC class II I E kappa Mouse mAb (Biotin) (Ab210121) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel – Mouse IgG (Biotin) (Ab180306).
MHC class II I E kappa Mouse mAb (Biotin) (Ab210121) - Flow Cytometry
Flow cytometry analysis of MHC class II I E kappa expression by rat splenocytes cells (red) compared to an isotype control (blue). Cells were stained with MHC class II I E kappa Mouse mAb (Biotin) (Ab210121) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
| Application | Dilution info |
|---|---|
| Flow Cytometry | ≤0.3μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume. |
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