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MHC class I/H-2Kb Mouse mAb (AF647) - Primary antibody, specific to MHC class I/H-2Kb

COA COA
    Application:
  • Flow Cytometry
  • Host species: Mouse
  • Reactive species: Mouse
  • Isotype: Mouse IgG2a
  • Clone Number: AF6-88.5.5.3
  • Conjugation: AF647
In stock
Item Number
Ab190297
Grouped product items
SKU Size
Availability
 Price Qty
Ab190297-10μg
10μg
3
$47.90
Ab190297-25μg
25μg
2
$95.90
Ab190297-100μg
100μg
1
$219.90
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Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (AF6-88.5.5.3); Mouse anti Mouse MHC class I/H-2Kb Antibody (AF647); Flow Cytometry

View related series
Click to view target information: HLA-A
Accession#: P01901 (13) AF647 (119) Flow Cytometry (1158) Gene ID: 14972 (17) H-2K (12) H-2K(d) (12) H2-D1 (13) H2-K (12) H2-K1 (17) K-f (12)

Basic Description

Product Name MHC class I/H-2Kb Mouse mAb (AF647) - Primary antibody, specific to MHC class I/H-2Kb
Synonyms K-f | H-2K | H2-K | H2-D1 | H-2K(d) | A-10 alpha chain | FLJ26655 | HLA class I histocompatibility antigen, A-1 alpha chain | HLA class I histocompatibility antigen, A-28 alpha chain | HLA class I histocompatibility antigen, A-9 alpha chain | HLA Class I
Specifications & Purity ExactAb™, Validated, Ex:650nm, Em:668nm, 0.5 mg/mL
Host species Mouse
Specificity MHC class I/H-2Kb
Immunogen C57BL/6 mouse splenocytes
Positive Control Flow: C57BL/6 mouse splenocytes.
Conjugation AF647
Excitation(nm) Red Laser(633nm)
Emission(nm) 668nm
Ex/Em(nm) Ex:650nm;Em:668nm
Grade Em:668nm, Ex:650nm, ExactAb™, Validated
Product Description

Mouse anti Mouse MHC class I/H-2Kb Antibody, Monoclonal (AF6-88.5.5.3), could be used for in vivo administration, Flow and so on.

Product Properties

Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Concentration 0.5 mg/mL
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

HLA-A Tbio HLA class I histocompatibility antigen, A-31 alpha chain (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

MHC class I/H-2Kb Mouse mAb (AF647) (Ab190297) - Flow Cytometry
Flow cytometry analysis of MHC class I/H-2Kb expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with MHC class I/H-2Kb Mouse mAb (AF647) (Ab190297) at 0.2μg/test for 1 hour at 4°C. Left panel - Mouse IgG (AF647) (Ab190077).

MHC class I/H-2Kb Mouse mAb (AF647) (Ab190297) - Flow Cytometry
Flow cytometry analysis of MHC class I/H-2Kb expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with MHC class I/H-2Kb Mouse mAb (AF647) (Ab190297) at 0.2μg/test for 1 hour at 4°C. Blue - Mouse IgG (AF647) (Ab190077). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

≤1.0 μg/test, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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