MCF7 Whole Cell Lysateused for WB various applications
Grade
Azide Free, Carrier Free
Product Description
Product Description：
The MCF7 whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process.
MCF-7 cells are a well-characterized estrogen receptor (ER) positive control cell line (cells are positive for cytoplasmic estrogen receptors) and therefore are a useful in vitro model of breast cancer to study the role of estrogen in breast cancer. Cells are also positive for cytokeratin and negative for desmin , endothelin , GFAP, neurofilament, vimentin.
Specifications & Purity
Carrier Free, Azide Free, 200μg, Mycoplasma free
Storage and Shipping
Application
WB
Shape
Lyophilized
Concentration
200μg, Mycoplasma free
Reconstitution
Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
Storage Temp
Store at -80°C
Shipped In
Ice chest + Ice pads
Images
MCF7 Whole Cell Lysate (cl155899)- Western Blot All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution
Lane 1: A549 Whole Cell Lysate (cl155906) Lane 2: MCF7 Whole Cell Lysate (cl155899) Lane 3: HeLa Whole Cell Lysate (cl155894) Lane 4: Hep G2 Whole Cell Lysate (cl155896) Lane 5: Raji Whole Cell Lysate (cl155907)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 42 kDa Observed band size: 42 kDa
MCF7 Whole Cell Lysate (cl155899) - Western Blot All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution
Lane 1: A549 Whole Cell Lysate (cl155906) Lane 2: MCF7 Whole Cell Lysate (cl155899) Lane 3: HeLa Whole Cell Lysate (cl155894) Lane 4: Hep G2 Whole Cell Lysate (cl155896) Lane 5: Raji Whole Cell Lysate (cl155907)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 29 kDa Observed band size: 29 kDa
MCF7 Whole Cell Lysate (cl155899) - Western Blot All lanes: Heat Shock Protein 60 antibody (HSP60) at 1/1000 dilution
Lane 1: A549 Whole Cell Lysate (cl155906) Lane 2: MCF7 Whole Cell Lysate (cl155899) Lane 3: HeLa Whole Cell Lysate (cl155894) Lane 4: Hep G2 Whole Cell Lysate (cl155896) Lane 5: Raji Whole Cell Lysate (cl155907)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 60 kDa Observed band size: 58 kDa
Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Find and download the COA for your product by matching the lot number on the packaging.
ZJ23F0500181