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Basic Description
| Product Name | Jurkat Whole Cell Lysate |
|---|---|
| Grade | Azide Free, Carrier Free |
| Product Description |
Product Description： The Jurkat whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process. Often this cell line is called 'JM' (Jurkat and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. Jurkat cells can be transfected and are therefore useful for studies of blood proto-oncogenes expression, apoptosis, and cell survival (e.g HIV). They produce IL-2 and can also be used for studies of differentiation. |
| Specifications & Purity | Carrier Free, Azide Free, 200μg, Mycoplasma free |
Storage and Shipping
| Shape | Lyophilized |
|---|---|
| Concentration | 200μg, Mycoplasma free |
| Storage Temp | Store at -80°C |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage. |
Images
Jurkat Whole Cell Lysate (cl155897) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution
Lane 1: Jurkat Whole Cell Lysate (cl155897)
Lane 2: MOLT-4 Whole Cell Lysate (cl155905)
Lane 3: LNCaP Whole Cell Lysate (cl155902)
Lane 4: Ramos Whole Cell Lysate (cl155908)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Jurkat Whole Cell Lysate (cl155897) - Western Blot
All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution
Lane 1: Jurkat Whole Cell Lysate (cl155897)
Lane 2: MOLT-4 Whole Cell Lysate (cl155905)
Lane 3: LNCaP Whole Cell Lysate (cl155902)
Lane 4: Ramos Whole Cell Lysate (cl155908)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
Jurkat Whole Cell Lysate (cl155897) - Western Blot
All lanes: Heat Shock Protein 60 antibody (HSP60) at 1/1000 dilution
Lane 1: Jurkat Whole Cell Lysate (cl155897)
Lane 2: MOLT-4 Whole Cell Lysate (cl155905)
Lane 3: LNCaP Whole Cell Lysate (cl155902)
Lane 4: Ramos Whole Cell Lysate (cl155908)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 60 kDa
Observed band size: 58 kDa
Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0700548 |
Certificate of Analysis | Jul 11, 2023 | cl155897 |
Documents & Articles
Subcellular Fractionation Protocols
Categories: Protocols
General Protocol for Western Blotting
Categories: Protocols
Solution Calculators
Reviews
Customer Reviews
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