Grouped product items

SKU

Size

Availability

Price

Qty

Ab110204-10μg

10μg

$39.90

Ab110204-50μg

50μg

$109.90

Ab110204-100μg

100μg

$169.90

Ab110204-1mg

1mg

Available within 8-12 weeks(?)

$499.90

Basic Description

Product Name	IL-27 p28 Mouse mAb - Primary antibody, specific to IL-27-A, Mouse IgG2a
Synonyms	p28 \| Il30 \| IL-27p28 \| IL-27 p28 subunit \| IL-27 subunit alpha \| IL27 \| IL-27 \| IL27A \| IL-27-A \| IL27-A \| IL27p28 \| IL30 \| interleukin 27 \| interleukin 30 \| interleukin-27 subunit alpha \| MGC71873 \| p28IL-27A \| EBI3 antibody \| IL 27 antibody \| IL 27 p28
Specifications & Purity	Carrier Free, Azide Free, Validated, ≥95%(SDS-PAGE), See COA
Host species	Mouse
Specificity	IL-27-A
Immunogen	Mouse IL-27
Conjugation	Unconjugated
Grade	Azide Free, Carrier Free, Validated
Product Description	Mouse anti Mouse IL-27-A Antibody, Monoclonal (MM27.7B1), could be used for Flow, in vivo IL-27 p28 neutralization, in vitro IL-27 p28 neutralization and so on. Application Flow: Use at an assay dependent concentration. in vivo IL-27 p28 neutralization: Use at an assay dependent concentration. in vitro IL-27 p28 neutralization: Use at an assay dependent concentration. Protein Function Cytokine with pro- and anti-inflammatory properties, that can regulate T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL-27 subunit p28 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replivation.

Product Properties

Isotype	Mouse IgG2a
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Shape	Liquid
Concentration	See COA
Storage Temp	Store at 2-8°C,Do not freeze
Shipped In	Wet ice
Stability And Storage	Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Associated Targets(Human)

IL27 Tbio Interleukin-27 subunit alpha (0 Activities)

Discover Target: IL27

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Application	Dilution info
Flow Cytometry	Use at an assay dependent concentration.
in vivo IL-27 p28 neutralization	Use at an assay dependent concentration
in vitro IL-27 p28 neutralization	Use at an assay dependent concentration

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0709068	Certificate of Analysis	Jul 31, 2024	Ab110204
ZJ24F0709067	Certificate of Analysis	Jul 31, 2024	Ab110204
ZJ24F0709066	Certificate of Analysis	Jul 31, 2024	Ab110204

Documents & Articles

Flow Cytometry Using Methanol Permeabilization

Categories: Protocols

Tags:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

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IL-27 p28 Mouse mAb - Primary antibody, specific to IL-27-A, Mouse IgG2a

Basic Description

Product Properties

Associated Targets(Human)

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Documents & Articles

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews

IL-27 p28 Mouse mAb - Primary antibody, specific to IL-27-A, Mouse IgG2a

Basic Description

Product Properties

Associated Targets(Human)

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Related Products

Documents & Articles

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews