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Basic Description
| Product Name | HeLa Whole Cell Lysate |
|---|---|
| Grade | Azide Free, Carrier Free |
| Product Description |
Product Description： The HeLa whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process. HeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro. |
| Specifications & Purity | Carrier Free, Azide Free, 200μg, Mycoplasma free |
Storage and Shipping
| Shape | Lyophilized |
|---|---|
| Concentration | 200μg, Mycoplasma free |
| Storage Temp | Store at -80°C |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage. |
Images
HeLa Whole Cell Lysate (cl155894) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution
Lane 1: A549 Whole Cell Lysate (cl155906)
Lane 2: MCF7 Whole Cell Lysate (cl155899)
Lane 3: HeLa Whole Cell Lysate (cl155894)
Lane 4: Hep G2 Whole Cell Lysate (cl155896)
Lane 5: Raji Whole Cell Lysate (cl155907)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
HeLa Whole Cell Lysate (cl155894) - Western Blot
All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution
Lane 1: A549 Whole Cell Lysate (cl155906)
Lane 2: MCF7 Whole Cell Lysate (cl155899)
Lane 3: HeLa Whole Cell Lysate (cl155894)
Lane 4: Hep G2 Whole Cell Lysate (cl155896)
Lane 5: Raji Whole Cell Lysate (cl155907)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
HeLa Whole Cell Lysate (cl155894) - Western Blot
All lanes: Heat Shock Protein 60 antibody (HSP60) at 1/1000 dilution
Lane 1: A549 Whole Cell Lysate (cl155906)
Lane 2: MCF7 Whole Cell Lysate (cl155899)
Lane 3: HeLa Whole Cell Lysate (cl155894)
Lane 4: Hep G2 Whole Cell Lysate (cl155896)
Lane 5: Raji Whole Cell Lysate (cl155907)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 60 kDa
Observed band size: 58 kDa
Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0500180 |
Certificate of Analysis | May 31, 2023 | cl155894 |
Documents & Articles
Subcellular Fractionation Protocols
Categories: Protocols
General Protocol for Western Blotting
Categories: Protocols
Solution Calculators
Reviews
Customer Reviews
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