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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab105966-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$79.90
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Ab105966-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$219.90
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Ab105966-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$359.90
|
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Ab105966-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,399.90
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mAb (A41S18); Mouse anti Human GNMT Antibody; WB, Flow; Unconjugated
| Product Name | GNMT Mouse mAb - Primary antibody, specific to GNMT, Mouse IgG2a |
|---|---|
| Synonyms | EC 2.1.1.20 antibody | Epididymis secretory sperm binding protein Li 182mP antibody | Glycine N methyltransferase antibody | Glycine N-methyltransferase antibody | Gnmt antibody | GNMT_HUMAN antibody | HEL S 182mP antibody | OTTHUMP00000016412 antibody | |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 0.5 mg/mL |
| Biochemical and Physiological Mechanisms | Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet a |
| Host species | Mouse |
| Specificity | GNMT |
| Immunogen | Recombinant protein of human GNMT. |
| Positive Control | WB: Mouse liver lysates. Flow: U-2 OS cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Mouse anti Human GNMT Antibody, Monoclonal (A41S18), could be used for WB, Flow and so on. |
| Isotype | Mouse IgG2a |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Shape | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
GNMT Mouse mAb (Ab105966) - Western Blot
All lanes: GNMT Mouse mAb (Ab105966) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 31 kDa
Exposure time: 20.8 s
GNMT Mouse mAb (Ab105966) - Flow Cytometry
Flow cytometric analysis of U-2 OS cells using GNMT Mouse mAb (Ab105966). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37℃ for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37℃ for 40 min. Isotype control antibody (blue line) was used under the same condition.
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 |
| Flow Cytometry | 1/10-1/50 |