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DUSP23 Mouse mAb - Primary antibody, specific to DUSP23

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DUSP23 Mouse mAb - Primary antibody, specific to DUSP23

Grade & Purity:

See COA

Application:

Flow Cytometry
IF/ICC
IHC
WB

Host species: Mouse
Reactive species: Human,Mouse,Rat
Isotype: Mouse IgG2a
Clone Number: OTI3C10
Conjugation: Unconjugated

In stock

Item Number

Ab100591

Grouped product items
SKU	Size	Availability	Price	Qty
Ab100591-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$79.90
Ab100591-50μl	50μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$229.90
Ab100591-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$359.90
Ab100591-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,899.90

mAb (OTI3C10); Mouse anti Human DUSP23 Antibody; WB, IHC, IF/ICC, Flow Cytometry; Unconjugated

View related series

Click to view target information: DUSP23

Accession#: Q9BVJ7 (1) DUSP23 (1) Flow Cytometry (1158) Gene ID: 54935 (1)

Basic Description

Product Name	DUSP23 Mouse mAb - Primary antibody, specific to DUSP23
Synonyms	Dual specificity phosphatase 23 \| Dual specificity protein phosphatase 23 \| DUS23_HUMAN \| Dusp23 \| DUSP25 \| EC 3.1.3.16 \| EC 3.1.3.48 \| FLJ20442 \| LDP 3 \| LDP-3 \| LDP3 \| Low molecular mass dual specificity phosphatase 3 \| MOSP \| RP11-190A12.1 \| VH1 like m
Specifications & Purity	ExactAb™, Validated, See COA
Biochemical and Physiological Mechanisms	Protein phosphatase that mediates dephosphorylation of proteins phosphorylated on Tyr and Ser/Thr residues. In vitro, it can dephosphorylate p44-ERK1 (MAPK3) but not p54 SAPK-beta (MAPK10) in vitro. Able to enhance activation of JNK and p38 (MAPK14).
Host species	Mouse
Specificity	DUSP23
Immunogen	Recombinant full length protein of human DUSP23 produced in HEK293T.
Positive Control	WB: A549 and MCF7 cell lysates. IHC: Human breast, kidney, carcinoma of kidney, adenocarcinoma of ovary, pancreas, endometrium, adenocarcinoma of endometrium, prostate, bladder, tonsil tissues. IF/ICC: COS7 cells transiently transfected with pCMV6-ENTRY D
Conjugation	Unconjugated
Grade	ExactAb™, Validated
Product Description	Mouse anti Human DUSP23 Antibody, Monoclonal (OTI3C10), could be used for WB, IHC, IF/ICC, Flow Cytometry and so on.

Product Properties

SDS-PAGE	150 kDa
Purification Method	Protein A purified
Shape	Liquid
Concentration	See COA
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

DUSP23 Tbio Dual specificity protein phosphatase 23 (0 Activities)

Discover Target: DUSP23

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Western Blot: DUSP23 Mouse mAb (Ab100591)

DUSP23 Mouse mAb (Ab100591) - Western Blot
All lanes: DUSP23 Mouse mAb (Ab100591) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/40000 dilution

Predicted band size: 16 kDa
Observed band size: 16 kDa
Exposure time: 66.0 s

IHC: DUSP23 Mouse mAb (Ab100591)

DUSP23 Mouse mAb (Ab100591) - IHC
Immunohistochemical staining of paraffinembedded Human breast tissue within the normal limits using DUSP23 Mouse mAb (Ab100591). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: DUSP23 Mouse mAb (Ab100591)

DUSP23 Mouse mAb (Ab100591) - IHC
Immunohistochemical staining of paraffinembedded Human Kidney tissue within the normal limits using DUSP23 Mouse mAb (Ab100591). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: DUSP23 Mouse mAb (Ab100591)

DUSP23 Mouse mAb (Ab100591) - IHC
Immunohistochemical staining of paraffinembedded Carcinoma of Human kidney tissue using DUSP23 Mouse mAb (Ab100591). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: DUSP23 Mouse mAb (Ab100591)

DUSP23 Mouse mAb (Ab100591) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of Human ovary tissue using using DUSP23 Mouse mAb (Ab100591). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: DUSP23 Mouse mAb (Ab100591)

DUSP23 Mouse mAb (Ab100591) - IHC
Immunohistochemical staining of paraffinembedded Human pancreas tissue within the normal limits using DUSP23 Mouse mAb (Ab100591). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: DUSP23 Mouse mAb (Ab100591)

DUSP23 Mouse mAb (Ab100591) - IHC
Immunohistochemical staining of paraffinembedded Human endometrium tissue within the normal limits using DUSP23 Mouse mAb (Ab100591). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: DUSP23 Mouse mAb (Ab100591)

DUSP23 Mouse mAb (Ab100591) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of Human endometrium tissue using DUSP23 Mouse mAb (Ab100591). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: DUSP23 Mouse mAb (Ab100591)

DUSP23 Mouse mAb (Ab100591) - IHC
Immunohistochemical staining of paraffinembedded Carcinoma of Human bladder tissue using DUSP23 Mouse mAb (Ab100591). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: DUSP23 Mouse mAb (Ab100591)

DUSP23 Mouse mAb (Ab100591) - IHC
Immunohistochemical staining of paraffinembedded Human prostate tissue within the normal limits using DUSP23 Mouse mAb (Ab100591). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: DUSP23 Mouse mAb (Ab100591)

DUSP23 Mouse mAb (Ab100591) - IHC
Immunohistochemical staining of paraffinembedded Human tonsil tissue within the normal limits using DUSP23 Mouse mAb (Ab100591). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IF/ICC: DUSP23 Mouse mAb (Ab100591)

DUSP23 Mouse mAb (Ab100591) - IF/ICC
DUSP23 Mouse mAb (Ab100591) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DUSP23.

Flow Cytometry: DUSP23 Mouse mAb (Ab100591)

DUSP23 Mouse mAb (Ab100591) - Flow Cytometry
Flow cytometric Analysis of HeLa cells, using DUSP23 Mouse mAb (Ab100591) (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: DUSP23 Mouse mAb (Ab100591)

DUSP23 Mouse mAb (Ab100591) - Flow Cytometry
Flow cytometric Analysis of Jurkat cells, using DUSP23 Mouse mAb (Ab100591) (Red), compared to a nonspecific negative control antibody (Blue).

Application

Application	Dilution info
WB	1/2000
Flow Cytometry	1/100
IF/ICC	1/100
IHC	1/150

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Documents & Articles

Flow Cytometry Using Methanol Permeabilization

Categories: Protocols

Tags:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

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