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Clostripain NB From Clostridium Histolyticum - Native activity: ≥ 5.0 U/mg (BAEE), high purity , CAS No.9028-00-6

COA

Grade & Purity:

EnzymoPure™

Native activity: ≥ 5.0 U/mg (BAEE)

In stock

Item Number

C279019

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SKU	Size	Availability	Price	Qty
C279019-200U	200U	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$1,030.90

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Metabolite (5307)

Basic Description

Product Name	Clostripain NB From Clostridium Histolyticum - Native activity: ≥ 5.0 U/mg (BAEE), high purity , CAS No.9028-00-6
Grade	EnzymoPure™
Product Description	Description Clostripain also known as Clostridiopeptidase B or Endoproteinase Arg-C is a proteinase that cleaves peptide bonds on the carboxyl side of arginine. This protein is a heterodimer composed of a heavy and a light chain that are held together via strong noncovalent forces. As a proteolytic enzyme it can be used for isolation of various cell types by tissue dissociation. Clostripain exists in two different forms, an oxidized, native condition and a reduced activated form that differ in enzymatic activity. The native clostripain can be activated with reducing agents (e.g. DTT, Cysteine) resulting in a higher activity of the enzyme. Application Clostripain NB is used in combination with Collagenase NB 1 and/or Neutral Protease NB, suitable for cell isolation from various tissue types. Specifications Contains chromatographically highly purified clostripain. Clostripain, native state activity: ≥ 5.0 U/mg (BAEE) Clostripain, activated activity: ≥ 50.0 U/mg (BAEE) Clostripain activity, native state, per vial: ≥ 200 U/vial (BAEE) Clostripain activity, activated, per vial: status EC 3.4.24.3 • Mr ca. 59 000 • CAS [9028-00-6] References and Definitions Unit definition: Clostripain, native state (BAEE assay): 1 Unit catalyzes the cleavage of 1 µmol Nα-Benzoyl-L-arginine ethyl ester per minute at 25 °C, pH 7.8. Clostripain, activated (BAEE assay): 1 Unit catalyzes the cleavage of 1 µmol Nα-Benzoyl-L-arginine ethyl ester per minute at 25 °C, pH 7.8. The enzyme is activated with 2.5 mM DTT. References 1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091 2. Kula et al. (1976) Biochem. Biophys. Res. Commun. 69, 389-396
Specifications & Purity	EnzymoPure™, Native activity: ≥ 5.0 U/mg (BAEE)

Storage and Shipping

Concentration	Native activity: ≥ 5.0 U/mg (BAEE)
Storage Temp	Store at -20°C
Shipped In	Ice chest + Ice pads
CAS	9028-00-6
Enzyme Commission Number	3.4.24.3
Unit definition	1 Unit catalyzes the cleavage of 1 µmol Nα-Benzoyl-L-arginine ethyl ester per minute at 25 °C, pH 7.8.

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