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Basic Description
| Product Name | Clostripain from Clostridium histolyticum - ≥50 units/mg dry weight, high purity , CAS No.9028-00-6 |
|---|---|
| Synonyms | Endoproteinase-Arg-C;Clostridiopeptidase B;Proteinase from Clostridium histolyticum |
| Grade | EnzymoPure™ |
| Product Description |
Clostripain (Endoproteinase-Arg-C) is a two chain proteinase associated with collagenase and isolated from Clostridium histolyticum. It is highly specific for the carboxyl peptide bond of arginine. Clostripain has a sulfhydryl requirement; it is activated by dithiothreitol, cysteine, or other sulfhydryl containing reagents. The presence of calcium ions is essential. The enzyme is inhibited by oxidizing agents and sulfhydryl reactants and by Co2+, Cu2+, Cd2+, and heavy metal ions. Citrate, borate, and Tris anions are less inhibitory. |
| Specifications & Purity | EnzymoPure™, ≥50 units/mg dry weight |
| Biochemical and Physiological Mechanisms | Clostripain from Clostridium histolyticum is composed of two polypeptide chains, with molecular masses of 41.7 kDa and 15.4 kDa. Clostripain has a highly restricted substrate specificity for Arg-Xaa peptide bonds. Therefore, clostripain has been explored |
Storage and Shipping
| Concentration | ≥50 units/mg dry weight |
|---|---|
| Storage Temp | Store at 2-8°C |
| Shipped In | Wet ice |
| CAS | 9028-00-6 |
| Enzyme Commission Number | 3.4.22.8 |
| Unit definition | One Unit hydrolyzes one micromole of N-benzoyl-L-arginine ethyl ester per minute at 25°C, pH 7.6, in the presence of dithiothreitol. |
Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 H2302489 |
Certificate of Analysis | Jul 10, 2023 | C128677 |
| B2321976 |
Certificate of Analysis | Dec 16, 2022 | C128677 |
| F2320018 |
Certificate of Analysis | Dec 16, 2022 | C128677 |
| K1816054 |
Certificate of Analysis | Sep 16, 2022 | C128677 |
| K1816052 |
Certificate of Analysis | Sep 16, 2022 | C128677 |
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