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CD95 Mouse mAb - Primary antibody, specific to CD95, Mouse IgG1

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CD95 Mouse mAb - Primary antibody, specific to CD95, Mouse IgG1

Grade & Purity:

See COA

Application:

Flow Cytometry

Host species: Mouse
Reactive species: Human
Isotype: Mouse IgG1
Clone Number: DX2
Conjugation: Unconjugated

In stock

Item Number

Ab006530

Grouped product items
SKU	Size	Availability	Price	Qty
Ab006530-10μg	10μg	10	$39.90
Ab006530-50μg	50μg	4	$109.90
Ab006530-100μg	100μg	2	$179.90
Ab006530-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$899.90

mAb (DX2); Mouse anti Human CD95 Antibody; Flow; Unconjugated

View related series

Click to view target information: FAS

Accession#:P25445 (7) CD95 (5) Fas (7) Flow Cytometry (1158) Gene ID:355 (7)

Basic Description

Product Name	CD95 Mouse mAb - Primary antibody, specific to CD95, Mouse IgG1
Synonyms	APO-1 \| FASTM \| ALPS1A \| Apo-1 antigen \| apoptosis antigen 1 \| Apoptosis-mediating surface antigen FAS \| APT1 \| APT1FASTM \| CD95 antigen \| CD95 \| CD95ALPS1A \| Fas (TNF receptor superfamily, member 6) \| Fas AMA \| Fas antigen \| Fas \| FAS1 \| FASLG receptor \|
Specifications & Purity	Carrier Free, ExactAb™, Azide Free, Validated, See COA
Host species	Mouse
Specificity	CD95
Immunogen	CD95 transfected L cells
Positive Control	Flow: Human peripheral blood lymphocytes.
Conjugation	Unconjugated
Grade	Azide Free, Carrier Free, ExactAb™, Validated
Product Description	Mouse anti Human CD95 Antibody, Monoclonal (DX2), could be used for Flow and so on. Application: Flow: 0.5 μg/100w cells Protein Function Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).

Product Properties

Isotype	Mouse IgG1
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein G purified
Shape	Liquid
Concentration	See COA
Storage Temp	Store at 2-8°C
Shipped In	Wet ice
Stability And Storage	Shipped at 4°C. Store at 4°C long term (12 months). Upon receipt, it is recommended to aliquot.

Associated Targets(Human)

FAS Tbio Tumor necrosis factor receptor superfamily member 6 (0 Activities)

Discover Target: FAS

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Images

Flow Cytometry: CD95 Mouse mAb (Ab006530)

CD95 Mouse mAb (Ab006530) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD95 with CD95 Mouse mAb (Ab006530) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD95 Mouse mAb (Ab006530)

CD95 Mouse mAb (Ab006530) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD95 (red) with CD95 Mouse mAb (Ab006530) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD95 Mouse mAb (Ab006530)

CD95 Mouse mAb (Ab006530) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood monocytes labelling CD95 with CD95 Mouse mAb (Ab006530) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD95 Mouse mAb (Ab006530)

CD95 Mouse mAb (Ab006530) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood monocytes labelling CD95 (red) with CD95 Mouse mAb (Ab006530) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD95 Mouse mAb (Ab006530)

CD95 Mouse mAb (Ab006530) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD95 (red) with CD95 Mouse mAb (Ab006530) at 0.1 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD95 Mouse mAb (Ab006530)

CD95 Mouse mAb (Ab006530) - Flow Cytometry
Flow Cytometry analysis of Raji cells labelling CD95 (red) with CD95 Mouse mAb (Ab006530) at 0.1 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD95 Mouse mAb (Ab006530)

CD95 Mouse mAb (Ab006530) - Flow Cytometry
Flow Cytometry analysis of Ramos cells labelling CD95 (red) with CD95 Mouse mAb (Ab006530) at 0.1 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD95 Mouse mAb (Ab006530)

CD95 Mouse mAb (Ab006530) - Flow Cytometry
Flow Cytometry analysis of A431 cells labelling CD95 (red) with CD95 Mouse mAb (Ab006530) at 0.1 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD95 Mouse mAb (Ab006530)

CD95 Mouse mAb (Ab006530) - Flow Cytometry
Flow Cytometry analysis of Hela cells labelling CD95 (red) with CD95 Mouse mAb (Ab006530) at 0.1 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application	Dilution info
Flow Cytometry	0.5µg/100w cells

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0404508	Certificate of Analysis	Apr 15, 2024	Ab006530
ZJ24F0404509	Certificate of Analysis	Apr 15, 2024	Ab006530
ZJ24F0404510	Certificate of Analysis	Apr 15, 2024	Ab006530

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