Accession#:P18337 (4) CD62L (10) Flow Cytometry (1158) Gene ID:20343 (5) L-Selectin (6) LAM-1 (3) LECAM-1 (3) LECAM1 (7) Ly-22 (3) Ly-m22 (3) Lyam-1 (3) LYAM1 (7)

Basic Description

Product Name	CD62L Rat mAb (Biotin) - Primary antibody, specific to CD62L, Rat IgG2a
Synonyms	CD62L antigen \| CD62L \| gp90-MEL \| hLHRc \| LAM1 \| LAM-1 \| LAM1LECAM1 \| LECAM1 \| LEU8 \| Leu-8 \| Leukocyte adhesion molecule 1 \| Leukocyte surface antigen Leu-8 \| Leukocyte-endothelial cell adhesion molecule 1 \| LNHR \| LNHRTQ1 \| LSEL \| L-Selectin \| LYAM1 \|
Specifications & Purity	ExactAb™, Validated, 0.5 mg/mL
Host species	Rat
Specificity	CD62L
Immunogen	C3H/eb mouse B lymphoma 38C-13
Positive Control	Flow: C57BL/6 mouse splenocytes.
Conjugation	Biotin
Grade	ExactAb™, Validated
Product Description	Application: Flow: ≤0.3 μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Product Properties

Isotype	Rat IgG2a
Light Chain Type	kappa
Purification Method	Protein G purified
Concentration	0.5 mg/mL
Storage Temp	Store at 2-8°C,Do not freeze
Shipped In	Wet ice
Stability And Storage	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

SELL Tchem L-selectin (0 Activities)

Discover Target: SELL

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

SELL Tchem Leukocyte adhesion molecule-1 (145 Activities)

Discover Target: SELL

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Images

Flow Cytometry: CD62L Rat mAb (Biotin) (Ab185155)

CD62L Rat mAb (Biotin) (Ab185155) - Flow Cytometry
Flow cytometry analysis of CD62L expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD62L Rat mAb (Biotin) (Ab185155) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application	Dilution info
Flow Cytometry	≤0.3μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

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1 results found

Lot Number	Certificate Type	Date	Item
ZJ24R0800923	Certificate of Analysis	Aug 16, 2024	Ab185155

Alternative Products

Documents & Articles

Immunoprecipitation Protocol for Native Proteins

Categories: Protocols

Tags:

Western blotting Immunoprecipitation Native Protein Non-denaturing Biotin Conjugation Streptavidin

Flow Cytometry Using Methanol Permeabilization

Categories: Protocols

Tags:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

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CD62L Rat mAb (Biotin) - Primary antibody, specific to CD62L, Rat IgG2a

Basic Description

Product Properties

Associated Targets(Human)

Mechanisms of Action

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Alternative Products

Documents & Articles

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews

CD62L Rat mAb (Biotin) - Primary antibody, specific to CD62L, Rat IgG2a

Basic Description

Product Properties

Associated Targets(Human)

Mechanisms of Action

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Related Products

Alternative Products

Documents & Articles

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews