Product Name	CD4 Rat mAb (PE) - Primary antibody, specific to CD4, Rat IgG2b
Synonyms	CD_antigen: CD4 \| CD4 antigen (p55) \| CD4 antigen \| CD4 molecule \| CD4 receptor \| CD4 \| CD4mut \| T-cell surface antigen T4/Leu-3 \| T-cell surface glycoprotein CD4 \| CD 4 antibody \| CD4 (L3T4) antibody \| CD4 antibody \| CD4 antigen (p55) antibody \| CD4 anti
Specifications & Purity	Validated, Ex:565nm, Em:575nm, 5μL/test
Host species	Rat
Specificity	CD4
Immunogen	Mouse CTL clone V4
Positive Control	Flow: Murine splenocytes.
Conjugation	PE
Excitation(nm)	Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)	575nm
Ex/Em(nm)	Ex:565nm;Em:575nm
Grade	Em:575nm, Ex:565nm, Validated
Product Description	Flow: 5μL/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume.

Product Properties

Isotype	Rat IgG2b
Purification Method	Protein G purified
Concentration	5μL/test
Storage Temp	Store at 2-8°C,Protected from light,Do not freeze
Shipped In	Wet ice
Stability And Storage	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

CD4 Tclin T-cell surface glycoprotein CD4 (0 Activities)

Discover Target: CD4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Images

Flow Cytometry: CD4 Rat mAb (PE) (Ab181111)

CD4 Rat mAb (PE) (Ab181111) - Flow Cytometry
Flow cytometry analysis of CD4 expression by mouse splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with CD4 Rat mAb (PE) (Ab181111) at 5μL/test for 1 hour at 4°C. Left panel - Rat IgG Isotype Control Antibody (PE) (Ab180312).

Flow Cytometry: CD4 Rat mAb (PE) (Ab181111)

CD4 Rat mAb (PE) (Ab181111) - Flow Cytometry
Flow cytometry analysis of CD4 expression by mouse splenocytes cells (red) compared to an isotype control (blue). Cells were stained with CD4 Rat mAb (PE) (Ab181111) at 5μL/test for 1 hour at 4°C. Blue - Rat IgG Isotype Control Antibody (PE) (Ab180312). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application	Dilution info
Flow Cytometry	5μl/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0606619	Certificate of Analysis	Jun 06, 2024	Ab181111
ZJ24F0606618	Certificate of Analysis	Jun 06, 2024	Ab181111
ZJ24F0606617	Certificate of Analysis	Jun 06, 2024	Ab181111

Alternative Products

Documents & Articles

Flow Cytometry Using Methanol Permeabilization

Categories: Protocols

Tags:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

Solution Calculators

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CD4 Rat mAb (PE) - Primary antibody, specific to CD4, Rat IgG2b

Basic Description

Product Properties

Associated Targets(Human)

Mechanisms of Action

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Alternative Products

Documents & Articles

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews

CD4 Rat mAb (PE) - Primary antibody, specific to CD4, Rat IgG2b

Basic Description

Product Properties

Associated Targets(Human)

Mechanisms of Action

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Related Products

Alternative Products

Documents & Articles

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews