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CD31 Rat mAb - Primary antibody, specific to CD31, Rat IgG2a

COA COA
    Application:
  • Animal Model
  • Blocking
  • Flow Cytometry
  • Functional Assay
  • IF/ICC
  • Host species: Rat
  • Reactive species: Mouse
  • Isotype: Rat IgG2a
  • Clone Number: 390
  • Conjugation: Unconjugated
In stock
Item Number
Ab094526
Grouped product items
SKU Size
Availability
 Price Qty
Ab094526-10μg
10μg
10
$29.90
Ab094526-50μg
50μg
4
$49.90
Ab094526-100μg
100μg
2
$79.90
Ab094526-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$439.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (390); Rat anti Mouse CD31 Antibody; Flow, ICC/IF, Intravital imaging, in vivo CD31 blocking; Unconjugated

View related series
Click to view target information: PECAM1
Accession#: Q08481 (11) CD31 (11) Flow Cytometry (1158) Gene ID: 18613 (12) PECAM1 (8)

Basic Description

Product Name CD31 Rat mAb - Primary antibody, specific to CD31, Rat IgG2a
Synonyms Cd31 | Pecam | adhesion molecule | CD31 antigen | CD31 | CD31/EndoCAM | EndoCAM | FLJ34100 | FLJ58394 | GPIIA' | PECA1 | PECAM1 | PECAM-1 | PECAM-1, CD31/EndoCAM | platelet endothelial cell adhesion molecule | platelet endothelial cell adhesion molecule-1
Specifications & Purity Carrier Free, Azide Free, Validated, ≥95%(SDS-PAGE), See COA
Host species Rat
Specificity CD31
Immunogen C3H/HeJ mouse hematopoietic progenitor cell line 3
Conjugation Unconjugated
Grade Azide Free, Carrier Free, Validated
Product Description

Rat anti Mouse CD31 Antibody, Monoclonal (390), could be used for Flow, ICC/IF, Intravital imaging, in vivo CD31 blocking and so on.
Application
Flow: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent concentration.
Intravital imaging: Use at an assay dependent concentration.
in vivo CD31 blocking: Use at an assay dependent concentration.
Protein Function
Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).

Product Properties

Isotype Rat IgG2a
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration See COA
Storage Temp Store at 2-8°C,Do not freeze
Shipped In Wet ice
Stability And Storage Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Associated Targets(Human)

PECAM1 Tbio Platelet endothelial cell adhesion molecule (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

CD31 Rat mAb (Ab094526) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD31 with CD31 Rat mAb (Ab094526) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).

CD31 Rat mAb (Ab094526) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD31 (red) with CD31 Rat mAb (Ab094526) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
IF/ICC

Use at an assay dependent concentration.
Flow Cytometry

Use at an assay dependent concentration.
Intravital imaging

Use at an assay dependent concentration.
in vivo CD31 blocking

Use at an assay dependent concentration.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ24F0707938 Certificate of Analysis Jul 08, 2024 Ab094526
ZJ24F0707937 Certificate of Analysis Jul 08, 2024 Ab094526
ZJ24F0707936 Certificate of Analysis Jul 08, 2024 Ab094526

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