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SKU | Size | Availability |
Price | Qty |
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Ab169225-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$16.90
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Ab169225-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$49.90
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Ab169225-100μl
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100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$72.90
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Ab169225-1ml
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1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$499.90
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mAb (CD01/3A10); Mouse anti Human CD25; Flow Cytometry, ELISA; Unconjugated
Product Name | CD25 Mouse mAb - Primary antibody, specific to Human CD25 |
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Synonyms | Interleukin 2 receptor alpha chain antibody | CD25 antibody | CD25 antigen antibody | IDDM10 antibody | IL 2 receptor alpha subunit antibody | IL-2 receptor subunit alpha antibody | IL-2-RA antibody | IL-2R subunit alpha antibody | IL2 RA antibody | IL2 R |
Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High Performance, ≥95%(SDS-PAGE), 1.0 mg/mL |
Biochemical and Physiological Mechanisms | Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells. |
Host species | Mouse |
Specificity | Human CD25 |
Immunogen | Recombinant protein corresponding to Human CD25 |
Positive Control | Flow Cytometry: Jurkat cells, Human peripheral blood lymphocytes. |
Conjugation | Unconjugated |
Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
Product Description |
Mouse anti Human CD25 Antibody, clone CD01/3A10, could be used for Flow Cytometry, ELISA and so on. |
Light Chain Type | kappa |
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SDS-PAGE | 150.0 KDa |
Purification Method | Protein A purified |
Concentration | 1.0 mg/mL |
Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
Shipped In | Ice chest + Ice pads |
Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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CD25 Mouse mAb (Ab169225) - Flow Cytometry
Flow Cytometry analysis of unstimulated (left) or 3-day PHA-stimulated (right) human peripheral blood lymphocytes labelling CD25 (red) with CD25 Mouse mAb (Ab169225) at 0.2 μg/mL for 1 hour at 4 °C and detected with AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (AF488). Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
CD25 Mouse mAb (Ab169225) - Flow Cytometry
Flow Cytometry analysis of unstimulated (left) or 3-day PHA-stimulated (right) human peripheral blood lymphocytes labelling CD25 (red) with CD25 Mouse mAb (Ab169225) at 0.2 μg/mL for 1 hour at 4 °C and detected with AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (AF488). Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
CD25 Mouse mAb (Ab169225) - Flow Cytometry
Flow Cytometry analysis of unstimulated (left) or 3-day PHA-stimulated (right) human peripheral blood lymphocytes labelling CD25 with CD25 Mouse mAb (Ab169225) at 0.2 μg/mL for 1 hour at 4 °C and detected with AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (AF488).
CD25 Mouse mAb (Ab169225) - Flow Cytometry
Flow Cytometry analysis of unstimulated (left) or 3-day PHA-stimulated (right) human peripheral blood lymphocytes labelling CD25 (red) with CD25 Mouse mAb (Ab169225) conjugated with biotin at 0.2 μg/mL for 1 hour at 4 °C and detected with SA-PE (rp156150). Blue - Isotype control, Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
CD25 Mouse mAb (Ab169225) - Flow Cytometry
Flow Cytometry analysis of unstimulated (left) or 3-day PHA-stimulated (right) human peripheral blood lymphocytes labelling CD25 (red) with CD25 Mouse mAb (Ab169225) conjugated with biotin at 0.2 μg/mL for 1 hour at 4 °C and detected with SA-PE (rp156150).
CD25 Mouse mAb (Ab169225) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells stained with CD25 Mouse mAb (Ab169225) (Red) at 1. 25 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG1-kappa, anti-E8L (Ab213557)was used as an isotype control (Blue).
Application | Dilution info |
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Flow Cytometry | 1/200 - 1/3000 |
Starting at $59.90
Starting at $49.90
Starting at $99.90