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CD25 Mouse mAb - Primary antibody, specific to Human CD25

    Application:
  • ELISA
  • Flow Cytometry
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG1
  • Clone Number: CD01/3A10
  • Conjugation: Unconjugated
In stock
Item Number
Ab169225
Grouped product items
SKU Size
Availability
Price Qty
Ab169225-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$16.90
Ab169225-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$49.90
Ab169225-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$72.90
Ab169225-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$499.90

mAb (CD01/3A10); Mouse anti Human CD25; Flow Cytometry, ELISA; Unconjugated

Basic Description

Product Name CD25 Mouse mAb - Primary antibody, specific to Human CD25
Synonyms Interleukin 2 receptor alpha chain antibody | CD25 antibody | CD25 antigen antibody | IDDM10 antibody | IL 2 receptor alpha subunit antibody | IL-2 receptor subunit alpha antibody | IL-2-RA antibody | IL-2R subunit alpha antibody | IL2 RA antibody | IL2 R
Specifications & Purity ExactAb™, Validated, Carrier Free, Azide Free, High Performance, ≥95%(SDS-PAGE), 1.0 mg/mL
Biochemical and Physiological Mechanisms Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Host species Mouse
Specificity Human CD25
Immunogen Recombinant protein corresponding to Human CD25
Positive Control Flow Cytometry: Jurkat cells, Human peripheral blood lymphocytes.
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Mouse anti Human CD25 Antibody, clone CD01/3A10, could be used for Flow Cytometry, ELISA and so on.

Product Properties

Light Chain Type kappa
SDS-PAGE 150.0 KDa
Purification Method Protein A purified
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Associated Targets(Human)

IL2RA Tclin Interleukin-2 receptor subunit alpha (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

CD25 Mouse mAb (Ab169225) - Flow Cytometry
Flow Cytometry analysis of unstimulated (left) or 3-day PHA-stimulated (right) human peripheral blood lymphocytes labelling CD25 (red) with CD25 Mouse mAb (Ab169225) at 0.2 μg/mL for 1 hour at 4 °C and detected with AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (AF488). Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

CD25 Mouse mAb (Ab169225) - Flow Cytometry
Flow Cytometry analysis of unstimulated (left) or 3-day PHA-stimulated (right) human peripheral blood lymphocytes labelling CD25 (red) with CD25 Mouse mAb (Ab169225) at 0.2 μg/mL for 1 hour at 4 °C and detected with AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (AF488). Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

CD25 Mouse mAb (Ab169225) - Flow Cytometry
Flow Cytometry analysis of unstimulated (left) or 3-day PHA-stimulated (right) human peripheral blood lymphocytes labelling CD25 with CD25 Mouse mAb (Ab169225) at 0.2 μg/mL for 1 hour at 4 °C and detected with AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (AF488).

CD25 Mouse mAb (Ab169225) - Flow Cytometry
Flow Cytometry analysis of unstimulated (left) or 3-day PHA-stimulated (right) human peripheral blood lymphocytes labelling CD25 (red) with CD25 Mouse mAb (Ab169225) conjugated with biotin at 0.2 μg/mL for 1 hour at 4 °C and detected with SA-PE (rp156150). Blue - Isotype control, Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

CD25 Mouse mAb (Ab169225) - Flow Cytometry
Flow Cytometry analysis of unstimulated (left) or 3-day PHA-stimulated (right) human peripheral blood lymphocytes labelling CD25 (red) with CD25 Mouse mAb (Ab169225) conjugated with biotin at 0.2 μg/mL for 1 hour at 4 °C and detected with SA-PE (rp156150).

CD25 Mouse mAb (Ab169225) - Flow Cytometry ​
Flow Cytometry analysis of Jurkat cells stained with CD25  Mouse mAb (Ab169225) (Red) at 1. 25 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG1-kappa, anti-E8L (Ab213557)was used as an isotype control (Blue).

Application

Application Dilution info
Flow Cytometry

1/200 - 1/3000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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