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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab156204-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$89.90
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Ab156204-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$219.90
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Ab156204-100μl
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100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$349.90
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Ab156204-1ml
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1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,199.90
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mAb (CD01/7F2); Mouse anti Human Caveolin-1; WB, Flow cytometry, IF/ICC, ELISA; Unconjugated
| Product Name | Caveolin-1 Mouse mAb - Primary antibody, specific to Caveolin-1, Mouse IgG1 |
|---|---|
| Synonyms | BSCL3 antibody | CAV antibody | CAV1 antibody | CAV1_HUMAN antibody | caveolae protein, 22 kD antibody | caveolin 1 alpha isoform antibody | caveolin 1 beta isoform antibody | Caveolin 1 caveolae protein 22kDa antibody | Caveolin-1 antibody | Caveolin1 an |
| Specifications & Purity | Carrier Free, ExactAb™, Azide Free, Validated, High performance, ≥95%(SDS-PAGE), 0.5 mg/mL |
| Biochemical and Physiological Mechanisms | May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T- |
| Host species | Mouse |
| Specificity | Caveolin-1 |
| Immunogen | Recombinant protein corresponding to Human Caveolin-1 |
| Positive Control | WB: HUVEC and C6 whole cell lysates Flow: HeLa cells IF/ICC: U-2 OS cells |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description |
Mouse anti Human Caveolin-1 Antibody, clone CD01/7F2, could be used for WB, Flow cytometry, IF/ICC, ELISA and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150.0 KDa |
| Purification Method | Protein A purified |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
Caveolin-1 Mouse mAb (Ab156204) - Western Blot
All lanes: Caveolin-1 Mouse Antibody (Ab156204) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 21 kDa
Exposure time: 30.0 s
Caveolin-1 Mouse mAb (Ab156204) - Flow Cytometry
Flow Cytometry analysis of HeLa cells stained with Caveolin-1 Mouse mAb (Ab156204) (Red) at 0.625 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG1 was used as an isotype control (Blue).
Caveolin-1 Mouse mAb (Ab191887) - ICC/IF
IF analysis of Profilin 1 (Green) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Caveolin-1 Mouse mAb (Ab156204) at 2.5 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
| Application | Dilution info |
|---|---|
| IF/ICC | 1/200 |
| Flow |
1/200 - 1/25,000
|
| WB | 1/1,000; Predicted molecular weight: 20.5 KDa |
Starting at $89.90
Starting at $59.90