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Enzymes

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  1. Recombinant Human ECE-2 Protein
      Species: Human    Accession #: EAW78275    
      Expression system: HEK293    Protein Tag: N-10His    Endotoxin Concentration: <0.1 EU/μg    
      Bioactivity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH. The specific activity is > 250 pmol/min/µg, as measured under the described conditions.    
    Synonyms: EC 3.4.24.71 | EC 3.4.24 | EC 3.4.24.71 | ECE2 | ECE-2 | endothelin converting enzyme 2 | endothelin-converting enzym...
  2. Recombinant Human Notum Protein
      Species: Human    Accession #: Q6P988    
      Expression system: HEK293    Protein Tag: C-His    Endotoxin Concentration: <0.1 EU/μg    
      Bioactivity: Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.1-0.6 μg/mL in the presence of 0.5 μg/mL of Recombinant Human Wnt-3a.    
    Synonyms: EC 3.- | notum pectinacetylesterase homolog (Drosophila) | Notum | protein notum homolog | NOTUM | hNOTUM | EC 3.- | ...
  3. Recombinant Mouse MMP-8 Protein
      Species: Mouse    Accession #: O70138    
      Expression system: HEK293    Protein Tag: C-His    Endotoxin Concentration: <0.1 EU/μg    
      Bioactivity: Measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2. The specific activity is >400 pmol/min/µg, as measured under the described conditions.    
    Synonyms: Matrix Metalloproteinase 8 | CLG1HNC | Collagenase 2 | EC 3.4.24 | EC 3.4.24.34 | matrix metallopeptidase 8 (neutroph...
  4. Recombinant Mouse YM1/Chitinase 3-like 3 Protein
      Species: Mouse    Accession #: O35744    
      Expression system: HEK293    Protein Tag: C-His    Endotoxin Concentration: <0.1 EU/μg    
      Bioactivity: Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. When 5 x 10^4 cells/well are added to Recombinant Mouse YM1/Chitinase 3-like 3 coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour incubation at 37 °C. The ED50 for this effect is 0.3-1.2 μg/mL.    
    Synonyms: AI505981 | CHI3L3 | Chitinase 3 like 3 | Chitinase 3-like 3 | ECF-L | YM1 | Chil3
  5. Recombinant Mouse Kallikrein 7 Protein
      Species: Mouse    Accession #: Q91VE3    
      Expression system: HEK293    Protein Tag: C-His    Endotoxin Concentration: <0.1 EU/μg    
      Bioactivity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is >70 pmol/min/μg, as measured under the described conditions.    
    Synonyms: EC 3.4.21 | EC 3.4.21.117 | hK7 | hSCCE | kallikrein 7 (chymotryptic, stratum corneum) | Kallikrein 7 | kallikrein-re...
  6. Recombinant Human Meprin beta Subunit/MEP1B Protein
      Species: Human    Accession #: Q16820    
      Expression system: HEK293    Protein Tag: C-10His    Endotoxin Concentration: <0.1 EU/μg    
      Bioactivity: Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2. The specific activity is >350 pmol/min/µg, as measured under the described conditions.    
    Synonyms: EC 3.4.24.63 | Endopeptidase-2 | Meprin B | N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta | PABA pe...
  7. 5' DNA/RNA Adenylase
      Species: Adenosylase gene of thermophilic bacteria    Accession #: 8UCH_A,A0A075LQ94    
      Expression system: E. coli    
      Bioactivity: One unit is the amount of enzyme required to catalyze the transfer of 1pmol of AMP to pDNA substrate in a total reaction volume of 20μl in 15 minutes at 65℃.    
    Synonyms: Single-Strand Nucleic Acid 5' Adenylase
  8. AlkB (RNA/DNA demethylase, Nuclease-free)
      Species: Escherichia coli (strain K12)    Accession #: P05050.1    
      Expression system: E. coli    
      Bioactivity: One unit (U) is the amount of AlkB that removes 90-100% of the m6A modifications in total RNA of HEK 293T cells in 20 ul of reaction mixture at 37℃ after 100minutes of incubation. Under the regular conditions, the enzymatic activity of 1 μg of AlkB is approximately 1 unit.    
  9. Antarctic Phosphatase (Thermosensitive)
      Species: Antarctic bacterium TAB5    Accession #: 2W5W_B,Q9KWY4    
      Expression system: E. coli    
      Bioactivity: 1 unit refers to the 5 'concave end produced by restriction endonuclease digestion at 37 ℃ for 30 minutes. The enzyme amount required for dephosphorylation of µ g pUC19 vector DNA. The dephosphorylation standard refers to the self linking reaction Can inhibit>95% of DNA re circularization and detect it by transforming E. coli cells.    
    Synonyms: Thermosensitive phosphatase
  10. Cre Recombinase
      Species: Escherichia phage P1    Accession #: Q5QBM7    
      Expression system: E. coli    
      Bioactivity: One unit is defined as the amount of enzyme required to achieve the maximum site-specific recombination of 250ng of control DNA in 30 minutes at 37ºC in a 50μl reaction system. The maximum recombination can be analyzed by agarose gel electrophoresis or by screening on the corresponding resistance plates after transformation of the reactions.    
    Synonyms: ‌Cyclization Recombination Enzyme | Type I topoisomerase | tyrosine recombinase
  11. Klenow Fragment, Exo-
      Species: E. coli    Accession #: 2KFZ_A,P00582    
      Expression system: E. coli    
      Bioactivity: One unit is the amount of enzyme required to catalyze the incorporation of 10nmol of deoxyribonucleotides (dNTPs) into a polynucleotide within 30 minutes at 37℃.    
    Synonyms: DNA polymerase I Fragment Mutant
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