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Recombinant Human Meprin beta Subunit/MEP1B Protein

  • Expression System: HEK293
  • Accession #: Q16820
  • Protein Tag: C-10His
  • Bioactivity: Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2. The specific activity is >350 pmol/min/µg, as measured under the described conditions.
  • Endotoxin Concentration: <0.1 EU/μg
In stock
Item Number
rp148698
Grouped product items
SKU Size
Availability
Price Qty
rp148698-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$359.90
rp148698-50μg
50μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$879.90
rp148698-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,319.90
rp148698-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$6,599.90

Animal Free, ≥95% (SDS-PAGE), Active, 293F, C-10*His tag, 21-593 aa & 23-593 aa

Basic Description

Product Name Recombinant Human Meprin beta Subunit/MEP1B Protein
Synonyms EC 3.4.24.63 | Endopeptidase-2 | Meprin B | N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta | PABA peptide hydrolase | PPH beta | EC 3.4.24.63 | endopeptidase-2 | MEP1B | meprin A subunit beta | meprin A, beta | Meprin B | Meprin beta Subu
Grade ActiBioPure™, Animal Free, Bioactive, Carrier Free
Product Description

Function
Membrane metallopeptidase that sheds many membrane-bound proteins. Exhibits a strong preference for acidic amino acids at the P1' position. Known substrates include: FGF19, VGFA, IL1B, IL18, procollagen I and III, E-cadherin, KLK7, gastrin, ADAM10, tenascin-C. The presence of several pro-inflammatory cytokine among substrates implicate MEP1B in inflammation. It is also involved in tissue remodeling due to its capability to degrade extracellular matrix components. Cleaves also the amyloid precursor protein/APP, thereby releasing neurotoxic amyloid beta peptides.

Specifications & Purity Animal Free, Carrier Free, Bioactive, ActiBioPure™, ≥95%(SDS-PAGE)
Bioactivity Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2. The specific activity is >350 pmol/min/µg, as measured under the described conditions.
Endotoxin Concentration <0.1 EU/μg
Expression System HEK293
Species Human
Amino Acids 21-593 aa & 23-593 aa
Sequence LATPENFDVDGGMDQDIFDINEGLGLDLFEGDIRLDRAQIRNSIIGEKYRWPHTIPYVLEDSLEMNAKGVILNAFERYRLKTCIDFKPWAGETNYISVFKGSGCWSSVGNRRVGKQELSIGANCDRIATVQHEFLHALGFWHEQSRSDRDDYVRIMWDRILSGREHNFNTYSDDISDSLNVPYDYTSVMHYSKTAFQNGTEPTIVTRISDFEDVIGQRMDFSDSDLLKLNQLYNCSSSLSFMDSCSFELENVCGMIQSSGDNADWQRVSQVPRGPESDHSNMGQCQGSGFFMHFDSSSVNVGATAVLESRTLYPKRGFQCLQFYLYNSGSESDQLNIYIREYSADNVDGNLTLVEEIKEIPTGSWQLYHVTLKVTKKFRVVFEGRKGSGASLGGLSIDDINLSETRCPHHIWHIRNFTQFIGSPNGTLYSPPFYSSKGYAFQIYLNLAHVTNAGIYFHLISGANDDQLQWPCPWQQATMTLLDQNPDIRQRMSNQRSITTDPFMTTDNGNYFWDRPSKVGTVALFSNGTQFRRGGGYGTSAFITHERLKSRDFIKGDDVYILLTVEDISHLNSHHHHHHHHHH
Protein Tag C-10His
Accession # Q16820
Predicted molecular weight 66 kDa
SDS-PAGE 75 - 95 kDa, under reducing conditions; 75 - 95 & 180 - 220 kDa, under non-reducing conditions.

Storage and Shipping

Shape Lyophilized
Reconstitution Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20~-80℃ for more than 1 year. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Human Meprin beta Subunit/MEP1B Protein (rp148698) - SDS-PAGE
2 μg/lane of Recombinant Human Meprin beta Subunit/MEP1B Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 75 - 95 kDa under reducing conditions and 75 - 95 & 180 - 220 kDa under non-reducing conditions.

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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