This is a demo store. No orders will be fulfilled.

PDPK1 Mouse mAb - Primary antibody, specific to PDPK1, IgG1

    Application:
  • ELISA
  • Flow Cytometry
  • IF/ICC
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse,Rat
  • Isotype: Mouse IgG1
  • Clone Number: 3H3D9
  • Conjugation: Unconjugated
In stock
Item Number
Ab121091
Grouped product items
SKU Size
Availability
Price Qty
Ab121091-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab121091-50μL
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$207.90
Ab121091-100μL
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$327.90
Ab121091-1mL
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,997.90

mAb (3H3D9); Mouse anti Human PDPK1 Antibody; WB, ICC, IF, ELISA, Flow; Unconjugated

Basic Description

Product Name PDPK1 Mouse mAb - Primary antibody, specific to PDPK1, IgG1
Synonyms EC 2.7.11.1 | hPDK1
Specifications & Purity ExactAb™, Validated, Carrier Free, 1.0mg/mL
Host species Mouse
Specificity PDPK1
Immunogen Purified recombinant fragment of human PDPK1 (AA: 457-556) expressed in E. Coli.
Positive Control WB: C6, K562, NIH/3T3 and MCF7 cell lysates. Flow: HeLa cells. ICC/IF: HeLa cells.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, Validated
Product Description

Application:
WB: 1/500 - 1/2000. Predicted molecular weight: 63 kDa.
IF/ICC: 1/200 - 1/1000
Flow: 1/200 - 1/400
ELISA: 1/10000

Protein Function
Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.

Product Properties

Isotype IgG1
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration 1.0mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon receipt, it is recommended to aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

Associated Targets(Human)

PDPK1 Tchem 3-phosphoinositide-dependent protein kinase 1 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

PDPK1 Mouse mAb (Ab121091) - Western Blot
All lanes: PDPK1 Mouse mAb (Ab121091) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 64 kDa
Observed band size: 51- 64 kDa
Exposure time: 113.7 s


PDPK1 Mouse mAb (Ab121091) - ICC/IF
Immunofluorescence analysis of HeLa cells using PDPK1 Mouse mAb (Ab121091) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with AF555 phalloidin.

PDPK1 Mouse mAb (Ab121091) - Flow Cytometry
Flow cytometric analysis of HeLa cells using PDPK1 Mouse mAb (Ab121091) (green) and negative control (red).

Application

Application Dilution info
IF/ICC

1/200-1/1000

Flow Cytometry

1/200-1/400

ELISA

1/10000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F0700736 Certificate of Analysis Aug 01, 2023 Ab121091
ZJ23F0700737 Certificate of Analysis Aug 01, 2023 Ab121091

Solution Calculators

Reviews

Customer Reviews

Shall we send you a message when we have discounts available?

Remind me later

Thank you! Please check your email inbox to confirm.

Oops! Notifications are disabled.