Determine the necessary mass, volume, or concentration for preparing a solution.
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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab121091-10μl
|
10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$69.90
|
|
|
Ab121091-50μL
|
50μl |
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
|
$207.90
|
|
|
Ab121091-100μL
|
100μl |
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
|
$327.90
|
|
|
Ab121091-1mL
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,997.90
|
|
mAb (3H3D9); Mouse anti Human PDPK1 Antibody; WB, ICC, IF, ELISA, Flow; Unconjugated
| Product Name | PDPK1 Mouse mAb - Primary antibody, specific to PDPK1, IgG1 |
|---|---|
| Synonyms | EC 2.7.11.1 | hPDK1 |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0mg/mL |
| Host species | Mouse |
| Specificity | PDPK1 |
| Immunogen | Purified recombinant fragment of human PDPK1 (AA: 457-556) expressed in E. Coli. |
| Positive Control | WB: C6, K562, NIH/3T3 and MCF7 cell lysates. Flow: HeLa cells. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Application: |
| Isotype | IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Shape | Liquid |
| Concentration | 1.0mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon receipt, it is recommended to aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
PDPK1 Mouse mAb (Ab121091) - Western Blot
All lanes: PDPK1 Mouse mAb (Ab121091) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 51- 64 kDa
Exposure time: 113.7 s
PDPK1 Mouse mAb (Ab121091) - ICC/IF
Immunofluorescence analysis of HeLa cells using PDPK1 Mouse mAb (Ab121091) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with AF555 phalloidin.
PDPK1 Mouse mAb (Ab121091) - Flow Cytometry
Flow cytometric analysis of HeLa cells using PDPK1 Mouse mAb (Ab121091) (green) and negative control (red).
| Application | Dilution info |
|---|---|
| IF/ICC |
1/200-1/1000
|
| Flow Cytometry |
1/200-1/400
|
| ELISA |
1/10000
|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Aug 01, 2023 | Ab121091 | |
| Certificate of Analysis | Aug 01, 2023 | Ab121091 |