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Kappa light chain Rat mAb (PE) - Primary antibody, specific to Kappa light chain

    Application:
  • Flow Cytometry
  • Host species: Rat
  • Reactive species: Mouse
  • Isotype: Rat IgG1
  • Clone Number: 187.1 (HB-58)
  • Conjugation: PE
In stock
Item Number
Ab190225
Grouped product items
SKU Size
Availability
Price Qty
Ab190225-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$26.90
Ab190225-25μg
25μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$54.90
Ab190225-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$159.90

mAb (187.1 (HB-58)); Rat anti Mouse Kappa light chain Antibody (PE); Flow Cytometry

Basic Description

Product Name Kappa light chain Rat mAb (PE) - Primary antibody, specific to Kappa light chain
Synonyms HCAK 1 antibody | HCAK1 antibody | Ig kappa chain C region antibody | IGKC antibody | IGKCD antibody | Immunoglobulin InV antibody | Immunoglobulin kappa constant antibody | Immunoglobulin kappa constant region antibody | Immunoglobulin kappa light chain
Specifications & Purity ExactAb™, Validated, 0.5 mg/mL
Host species Rat
Specificity Kappa light chain
Immunogen Mouse IgG2b Isotype control antibody clone MPC-11
Positive Control Flow Cytometry: C57BL/6 mouse splenocytes
Conjugation PE
Excitation(nm) Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm) 575nm
Ex/Em(nm) Ex:565nm;Em:575nm
Grade ExactAb™, Validated
Product Description

Rat anti Mouse Kappa light chain Antibody, Monoclonal (187.1 (HB-58)), could be used for Flow Cytometry.

Product Properties

Purification Method Protein G purified
Concentration 0.5 mg/mL
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

IGKC Tdark Immunoglobulin kappa constant (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Kappa light chain Rat mAb (PE) (Ab190225) - Flow Cytometry
Flow cytometry analysis of kappa light chain expression by mouse splenocytes (right panel) compared to an isotype control (left panel). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with Kappa light chain Rat mAb (PE) (Ab190225) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE) (Ab180312).

Kappa light chain Rat mAb (PE) (Ab190225) - Flow Cytometry
Flow cytometry analysis of kappa light chain expression by mouse splenocytes (red) compared to an isotype control (blue). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with Kappa light chain Rat mAb (PE) (Ab190225) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (PE) (Ab180312). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

≤1.0μg/ test

For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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