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CD99 Mouse mAb - Primary antibody, specific to CD99, Mouse IgG2a

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CD99 Mouse mAb - Primary antibody, specific to CD99, Mouse IgG2a

Grade & Purity:

See COA

Application:

Flow Cytometry

Host species: Mouse
Reactive species: Human
Isotype: Mouse IgG2a
Clone Number: HI156
Conjugation: Unconjugated

In stock

Item Number

Ab095548

Grouped product items
SKU	Size	Availability	Price	Qty
Ab095548-10μg	10μg	10	$29.90
Ab095548-50μg	50μg	4	$69.90
Ab095548-100μg	100μg	2	$109.90
Ab095548-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$899.90

mAb (HI156); Mouse anti Human CD99 Antibody; Flow; Unconjugated

View related series

Click to view target information: CD99

Accession#:P14209 (3) CD99 (3) Flow Cytometry (1158) Gene ID:4267 (3) MIC2X (1)

Basic Description

Product Name	CD99 Mouse mAb - Primary antibody, specific to CD99, Mouse IgG2a
Synonyms	12E7 \| E2 antigen \| Protein MIC2 \| T-cell surface glycoprotein E2 \| MIC2X \| 12E7 \| CD99 antigenY homolog \| CD99 molecule \| CD99 \| E2 antigen \| HBA71 \| MIC2 (monoclonal 12E7) \| MIC2 \| MIC2Y \| MSK5X \| pilr-1 \| PILR-L \| Protein MIC2 \| surface antigen MIC2 \|
Specifications & Purity	Carrier Free, ExactAb™, Validated, See COA
Host species	Mouse
Specificity	CD99
Immunogen	Human endothelial cells
Positive Control	Flow: Human peripheral blood lymphocytes.
Conjugation	Unconjugated
Grade	Carrier Free, ExactAb™, Validated
Product Description	Mouse anti Human CD99 Antibody, Monoclonal (HI156), could be used for Flow and so on. Application: Flow: < 0.5 μg/100w cells Protein Function Involved in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes.

Product Properties

Isotype	Mouse IgG2a
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Shape	Liquid
Concentration	See COA
Storage Temp	Store at 2-8°C
Shipped In	Wet ice
Stability And Storage	Shipped at 4°C. Store at 4°C long term (36 months). Upon receipt, it is recommended to aliquot.

Associated Targets(Human)

CD99 Tbio CD99 antigen (0 Activities)

Discover Target: CD99

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Flow Cytometry: CD99 Mouse mAb (Ab095548)

CD99 Mouse mAb (Ab095548) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD99 with CD99 Mouse mAb (Ab095548) conjugated with biotin (right panel) at 2.0 μg/mL for 1 hour at 4°C, compared with Mouse IgG (Biotin) (Ab170221) - Isotype Control (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD99 Mouse mAb (Ab095548)

CD99 Mouse mAb (Ab095548) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD99 (red) with CD99 Mouse mAb (Ab095548) conjugated with biotin at 2.0 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, Mouse IgG (Biotin) (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD99 Mouse mAb (Ab095548)

CD99 Mouse mAb (Ab095548) - Flow Cytometry
Flow Cytometry analysis of A549 cells labelling CD99 (red) with CD99 Mouse mAb (Ab095548) at 5.0 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD99 Mouse mAb (Ab095548)

CD99 Mouse mAb (Ab095548) - Flow Cytometry
Flow Cytometry analysis of A431 cells labelling CD99 (red) with CD99 Mouse mAb (Ab095548) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD99 Mouse mAb (Ab095548)

CD99 Mouse mAb (Ab095548) - Flow Cytometry
Flow Cytometry analysis of HeLa cells labelling CD99 (red) with CD99 Mouse mAb (Ab095548) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD99 Mouse mAb (Ab095548)

CD99 Mouse mAb (Ab095548) - Flow Cytometry
Flow Cytometry analysis of K562 cells labelling CD99 (red) with CD99 Mouse mAb (Ab095548) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application	Dilution info
Flow	< 0.5µg/100w cells

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0506083	Certificate of Analysis	May 24, 2024	Ab095548
ZJ24F0506082	Certificate of Analysis	May 24, 2024	Ab095548
ZJ24F0506081	Certificate of Analysis	May 24, 2024	Ab095548

Alternative Products

Documents & Articles

Flow Cytometry Using Methanol Permeabilization

Categories: Protocols

Tags:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

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