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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab185392-10μg
|
10μg |
2
|
$29.90
|
|
|
Ab185392-25μg
|
25μg |
3
|
$59.90
|
|
|
Ab185392-100μg
|
100μg |
1
|
$139.90
|
|
mAb (1D3); Rat anti Mouse CD19 Antibody (Biotin); Flow
| Product Name | CD19 Rat mAb(Biotin) - Primary antibody, specific to CD19, Rat IgG2a |
|---|---|
| Synonyms | Biotin Anti-Mouse CD19 Antibody[1D3] | Biotin Anti-CD19 antibody [1D3] | Biotin Rat Anti-Mouse CD19 | Biotin anti-mouse CD19 Antibody | T-cell surface antigen Leu-12 antibody | MGC12802 antibody | MGC109570 antibody | Leu12 antibody | Leu-12 antibody | Le |
| Specifications & Purity | ExactAb™, Validated, 0.5 mg/mL |
| Host species | Rat |
| Specificity | CD19 |
| Immunogen | Transfected k562 cells expressing the extracellular domain of murine CD19. |
| Positive Control | Flow: Mouse splenocytes. |
| Conjugation | Biotin |
| Grade | ExactAb™, Validated |
| Product Description |
Flow: ≤0.3 μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume. |
| Isotype | Rat IgG2a |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at 2-8°C,Protected from light,Do not freeze |
| Shipped In | Wet ice |
| Stability And Storage | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
CD19 Rat mAb (Biotin) (Ab185392) - Flow Cytometry
Flow cytometry analysis of CD19 expression by mouse splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with CD19 Rat mAb (Biotin) (Ab185392) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG Isotype Control Antibody (Biotin) (Ab180310).
CD19 Rat mAb (Biotin) (Ab185392) - Flow Cytometry
Flow cytometry analysis of CD19 expression by mouse splenocytes cells (red) compared to an isotype control (blue). Cells were stained with CD19 Rat mAb (Biotin) (Ab185392) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.
| Application | Dilution info |
|---|---|
| Flow Cytometry | ≤0.3μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume. |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Aug 02, 2024 | Ab185392 | |
| Certificate of Analysis | Aug 02, 2024 | Ab185392 | |
| Certificate of Analysis | Aug 02, 2024 | Ab185392 | |
| Certificate of Analysis | Jul 18, 2024 | Ab185392 |
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