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CD14 Mouse mAb - Primary antibody, specific to CD14

    Application:
  • Flow Cytometry
  • WB
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG1
  • Clone Number: OTI4H4
  • Conjugation: Unconjugated
In stock
Item Number
Ab093874
Grouped product items
SKU Size
Availability
Price Qty
Ab093874-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$59.90
Ab093874-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$169.90
Ab093874-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$249.90
Ab093874-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,999.90

mAb (OTI4H4); Mouse anti Human CD14 Antibody; WB, Flow Cytometry; Unconjugated

Basic Description

Product Name CD14 Mouse mAb - Primary antibody, specific to CD14
Synonyms CD14 antigen | CD14 molecule | CD14 | monocyte differentiation antigen CD14 | Myeloid cell-specific leucine-rich glycoprotein | CD 14 antibody | CD_antigen=CD14 antibody | CD14 antibody | CD14 antigen antibody | CD14 molecule antibody | CD14_HUMAN antibod
Specifications & Purity ExactAb™, Validated, See COA
Biochemical and Physiological Mechanisms Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molec
Host species Mouse
Specificity CD14
Immunogen Recombinant protein of human CD14 (AA 20-367)
Positive Control WB: HL60 and A549 cell lysates. Flow Cytometry: HEK293T cells transfected with pCMV6-ENTRY CD14.
Conjugation Unconjugated
Grade ExactAb™, Validated
Product Description

Mouse anti Human CD14 Antibody, Monoclonal (OTI4H4), could be used for WB, Flow Cytometry and so on.

Product Properties

SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

CD14 Tbio Monocyte differentiation antigen CD14 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

CD14 Mouse mAb (Ab093874) - Western Blot
All lanes: CD14 Mouse mAb (Ab093874) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution

Predicted band size: 40 kDa
Observed band size: 51 kDa
Exposure time: 15.0 s

CD14 Mouse mAb (Ab093874) - Western Blot
All lanes: CD14 Mouse mAb (Ab093874) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/5000 dilution

Predicted band size: 40 kDa
Observed band size: 51 kDa
Exposure time: 7.8 s

CD14 Mouse mAb (Ab093874) - Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by CD14 Mouse mAb (Ab093874), and then analyzed by flow cytometry (1/100).

Application

Application Dilution info
Flow Cytometry

1/100

WB

1/500 - 1/1000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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