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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab093874-10μl
|
10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$59.90
|
|
|
Ab093874-50μl
|
50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$169.90
|
|
|
Ab093874-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$249.90
|
|
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Ab093874-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$1,999.90
|
|
mAb (OTI4H4); Mouse anti Human CD14 Antibody; WB, Flow Cytometry; Unconjugated
| Product Name | CD14 Mouse mAb - Primary antibody, specific to CD14 |
|---|---|
| Synonyms | CD14 antigen | CD14 molecule | CD14 | monocyte differentiation antigen CD14 | Myeloid cell-specific leucine-rich glycoprotein | CD 14 antibody | CD_antigen=CD14 antibody | CD14 antibody | CD14 antigen antibody | CD14 molecule antibody | CD14_HUMAN antibod |
| Specifications & Purity | ExactAb™, Validated, See COA |
| Biochemical and Physiological Mechanisms | Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molec |
| Host species | Mouse |
| Specificity | CD14 |
| Immunogen | Recombinant protein of human CD14 (AA 20-367) |
| Positive Control | WB: HL60 and A549 cell lysates. Flow Cytometry: HEK293T cells transfected with pCMV6-ENTRY CD14. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Validated |
| Product Description |
Mouse anti Human CD14 Antibody, Monoclonal (OTI4H4), could be used for WB, Flow Cytometry and so on. |
| SDS-PAGE | 150 kDa |
|---|---|
| Purification Method | Protein G purified |
| Shape | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
CD14 Mouse mAb (Ab093874) - Western Blot
All lanes: CD14 Mouse mAb (Ab093874) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution
Predicted band size: 40 kDa
Observed band size: 51 kDa
Exposure time: 15.0 s
CD14 Mouse mAb (Ab093874) - Western Blot
All lanes: CD14 Mouse mAb (Ab093874) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/5000 dilution
Predicted band size: 40 kDa
Observed band size: 51 kDa
Exposure time: 7.8 s
CD14 Mouse mAb (Ab093874) - Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by CD14 Mouse mAb (Ab093874), and then analyzed by flow cytometry (1/100).
| Application | Dilution info |
|---|---|
| Flow Cytometry | 1/100 |
| WB | 1/500 - 1/1000 |
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