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CD127 Rat mAb (FITC) - Primary antibody, specific to CD127, Rat IgG2a

COA COA
    Application:
  • Flow Cytometry
  • Host species: Rat
  • Reactive species: Mouse
  • Isotype: Rat IgG2a
  • Clone Number: A7R34
  • Conjugation: FITC
In stock
Item Number
Ab185389
Grouped product items
SKU Size
Availability
 Price Qty
Ab185389-10μg
10μg
7
$49.90
Ab185389-25μg
25μg
4
$89.90
Ab185389-100μg
100μg
3
$289.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (A7R34); Rat anti Mouse CD127 Antibody (FITC); Flow

View related series
Click to view target information: IL7R
Accession#:P16872 (5) CD127 (13) FITC (181) Flow Cytometry (1158) Gene ID:16197 (5) IL-7Ralpha (4) IL7R (13)

Basic Description

Product Name CD127 Rat mAb (FITC) - Primary antibody, specific to CD127, Rat IgG2a
Synonyms FITC Anti-Mouse CD127/IL-7RA Antibody[A7R34] | CD127 Monoclonal Antibody (A7R34), FITC | FITC Rat Anti-Mouse CD127 | FITC anti-mouse CD127 (IL-7Rα) Antibody | Interleukin-7 receptor subunit alpha antibody | Interleukin 7 receptor isoform H5 6 antibody | I
Specifications & Purity ExactAb™, Validated, Ex:498nm, Em:517nm, 0.5 mg/mL
Host species Rat
Specificity CD127
Immunogen IL-7Ra-IgG1 fusion protein.
Positive Control Flow: Murine splenocytes.
Conjugation FITC
Excitation(nm) Blue Laser(488nm)
Emission(nm) 517nm
Ex/Em(nm) Ex:498nm;Em:517nm
Grade Em:517nm, Ex:498nm, ExactAb™, Validated
Product Description

Application:
Flow: ≤1.0 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Product Properties

Isotype Rat IgG2a
Light Chain Type kappa
Purification Method Protein G purified
Concentration 0.5 mg/mL
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

IL7R Tbio Interleukin-7 receptor subunit alpha (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

CD127 Rat mAb (FITC) (Ab185389) - Flow Cytometry
Flow cytometry analysis of CD127 expression by mouse splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with CD127 Rat mAb (FITC) (Ab185389) at 0.2μg/test for 1 hour at 4°C. Left panel - Rat IgG Isotype Control Antibody (FITC) (Ab180311).

CD127 Rat mAb (FITC) (Ab185389) - Flow Cytometry
Flow cytometry analysis of CD127 expression by mouse splenocytes cells (red) compared to an isotype control (blue). Cells were stained with CD127 Rat mAb (FITC) (Ab185389) at 0.2μg/test for 1 hour at 4°C. Blue - Rat IgG Isotype Control Antibody (FITC) (Ab180311). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

≤1.0μg/ test

For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot Number Certificate Type Date Item
ZJ24F0708558 Certificate of Analysis Jul 22, 2024 Ab185389
ZJ24F0708557 Certificate of Analysis Jul 22, 2024 Ab185389
ZJ24F0708556 Certificate of Analysis Jul 22, 2024 Ab185389
ZJ24R0700804 Certificate of Analysis Jul 12, 2024 Ab185389

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